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Analytical Chemistry

Analytical chemistry is the subject for the method and basic principle of studying and identifying of the composition, status, structure of matter as well as determination of related content. It is an important branch of chemistry subject. Analytical chemistry plays an important role in not only its own development but also in various fields related to the chemistry. We can say that all the practice of any human activity involving chemical phenomena is inseparable from analytical chemistry. Now, people have developed various kinds of different analytical methods, which can be classified based on an analysis task, analysis object, the basis of the analysis, requirement of the analysis and sample dosage.

According to the analysis tasks, it can be divided into qualitative analysis, quantitative analysis and structural analysis. Task of qualitative analysis is to identify the elements, radicals, functional groups or compounds that constituting the substances; the task of the quantitative analysis is to determine the content of the related components in the sample; the task of structural analysis is to study the molecular structure or crystal structure of the material.

(1) According to the analysis objects, it can be divided into organic and inorganic analysis; the object for the inorganic analysis is inorganic substance; the object of organic analysis is organic substance. In the inorganic analysis, it is generally required to determine what elements, ions, radicals or compounds that constitutes the sample and measure the percentage of each component; and sometimes it is also necessary for determination of the crystal structure; in the organic analysis, it not only requires the identification of the constituent elements, but also, more importantly, should do the structure analysis and functional group analysis.
(2) According to whether the analysis is based on the physical properties or chemical properties of the substance, it can be divided into instrumental analysis and chemical analysis. Depending on the specific requirements, it can be divided into routine analysis, rapid analysis and arbitration Analysis. Routine analysis refers to the general daily laboratory production analysis, also known as conventional analysis; rapid analysis is a kind of routine analysis and is mainly applied to the control of the production process, demanding the report of the results in the shortest possible time with the error generally being allowed to be greater; arbitration analysis is needed when there is controversy in the analysis results from different institute, demanding related department to conduct accurate analysis using specific method in order to determine the accuracy of the results of the original analysis.
(3) According to the amount of the sample, it can be generally divided into constant (> 0.1g), semi-micro (0.01 ~ 0.1g) and trace (1 ~ 10mg) analysis.
(4) In the inorganic qualitative chemical analysis, people generally apply semi-micro method while people generally apply constant analysis in the quantitative chemical analysis. According to the relative amounts of the analyzed components contained in the sample, it is also roughly divided into constant component analysis (> 1%), minor component analysis (0.01 to 1%) and trace components analysis (<0.01%). For the analysis of some trace amount of components contained in some kinds of complicated mixture and some substances, it is necessary to perform separation and enrichment. This produces a series of separation techniques, such as extraction, distillation, ion exchange, chromatography, sedimentation and flotation separation, these chemical separation techniques are an integral part of the analysis.

Environmental Analytical Chemistry
Environmental Analytical Chemistry is briefly referred to environmental analysis. It is a kind of subject to study the types, components of pollutants in the environment as well as how to perform qualitative and quantitative analysis on the chemical contaminants in the environment. It is a branch of environmental chemistry.

Environmental analytical chemistry emerged, developed and improved during the process of solving environmental problems. In 1950s, the public nuisance disease occurred in Japan had alerted the whole world. In order to find the cause of public nuisance disease, after experiencing as long as 11 years, later, the chemists of environmental analysis had applied light spectrum and identified that the river in Itai-itai disease area contained harmful elements such as lead, cadmium, arsenic and so on. Further by means of tracking element analysis of the soil and food in the disease area, people had found high lead and cadmium content. Later, people had further conducted spectral quantitative analysis on the body and bone of the patients in the disease area and found that the bone ashes contained alarmingly high content of zinc, lead and cadmium. To determine the causative agent, people further incorporated zinc, lead and cadmium into the food for feeding animals and conduct trace elemental analysis for animals and confirmed the serious harm of cadmium on the bone, revealing the cause of the Itai-itai disease. The development of modern science, especially the development of modern chemistry, physics, mathematics, electronics, biology, as well as the emergence of accurate, reliable, sensitive, selective, rapid, simple environmental pollution analysis technology and automation equipment, has been resulting in the maturation of environmental analytical chemistry. Environmental analytical chemistry now has penetrated into all areas of the entire environmental science subject. It is the most effective means of access to environmental information quality.
The objects of the environmental analytical chemistry research are quite complicated, including air, water, soil, sediment, minerals, waste, animals, plants, food, and human tissue. The content of chemical elements or compound to be determined in the environmental analytic chemistry is very low, with the absolute content being within the level of 10-6 to 10-12 grams.


The analysis technology in the environmental analytical chemistry is developing towards the direction of continuous automation, computerization and joint combination of various methods and instruments. Currently applied automatic analysis methods include colorimetric analysis, ion selective electrode, x-ray fluorescence spectroscopy, atomic absorption spectroscopy, polarography, gas chromatography, liquid chromatography and flow injection analysis. Laser, as the light source of analytical chemistry technique, has also been applied. Since the laser analysis has properties of high resolution, high sensitivity, long-range and short-term, the laser technology will play a pivotal role in the development of environmental analytical chemistry.

With the deepened development of environmental science, environmental analytical chemistry is often demanded for trace levels and ultra-trace-level detection and analysis, therefore, high sensitivity. Thus study of analysis methods of high sensitivity, good selectivity, rapid trace and ultra trace will become the major development direction for environmental analysis in the near future.

Qualitative Analysis of Chemistry
Qualitative analytic chemistry is the subject to identify the chemical elements and atoms groups contained in the sample. It is a branch subject of the analysis chemistry. Its purpose is to ascertain the chemical composition of the research object (specimen).
The major research content of the qualitative analytic chemistry includes:

1 the tested samples were analyzed separately. Namely take part of the sample and use exclusive reaction to detect a desire detection component.
2 systematic analysis of the samples. This means successively apply a few selective reactions for gradual separation of the ions followed by separation of each group until separating to only one substance and finally apply confirming reaction to ascertain the existence of this substance. The most famous cation system analysis method is H2S system. In recent years, due to the use of advanced equipment, qualitative analysis has also rapidly developed together with multivariate analysis and has also become an important direction for analytical chemistry.

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Structure Chemical Name CAS MF
Tofacitinib Impurity 69 Tofacitinib Impurity 69
Benzenamine, 3-(2-methyl-1H-imidazol-1-yl)-5-(trifluoromethyl)- Benzenamine, 3-(2-methyl-1H-imidazol-1-yl)-5-(trifluoromethyl)- 641571-06-4 C11H10F3N3
Ciprofloxacin EP Impurity E Ciprofloxacin EP Impurity E 879005-96-6 C16H20FN3O
ethyl 2-(3-cyano-4-propoxyphenyl)-4-methylthiazole-5-
carboxylate ethyl 2-(3-cyano-4-propoxyphenyl)-4-methylthiazole-5- carboxylate C17H18N2O3S
(2S,5R,6S)-6-bromo-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid Diphenylmethyl Ester (2S,5R,6S)-6-bromo-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid Diphenylmethyl Ester 74189-25-6 C21H20BrNO3S
Trelagliptin Impurity 5 Trelagliptin Impurity 5 2087874-92-6 C22H24FN5O5
9H-Purin-6-amine, 9-beta-D-arabinofuranosyl-2-methoxy- 9H-Purin-6-amine, 9-beta-D-arabinofuranosyl-2-methoxy- 51742-84-8 C11H15N5O5
1616710-52-1 1616710-52-1 1616710-52-1 C27H34N2O6
Labetalol EP impurity E Labetalol EP impurity E
1-Propanol, 3-(2-methoxy-5-nitrophenoxy)- 1-Propanol, 3-(2-methoxy-5-nitrophenoxy)- 1156068-41-5 C10H13NO5
Bendamustine Related Impurity 22 Bendamustine Related Impurity 22
Bendamustine Related Impurity 9 Bendamustine Related Impurity 9
Edoxaban Impurity 20 (1R,2R,4S) Edoxaban Impurity 20 (1R,2R,4S) 1928729-31-0 C16H29N3O7
Edoxaban Impurity 40 Edoxaban Impurity 40 2081883-52-3 C14H27N3O3
Everolimus Impurity 2 Everolimus Impurity 2
Levosimendan Impurity 2 (Mixture ofZandEIsomers) Levosimendan Impurity 2 (Mixture ofZandEIsomers)
N-(2,3-Dichloro-phenyl)-N'-[2-(2,3-dichloro-phenylamino)-ethyl]-ethane-1,2-diamine N-(2,3-Dichloro-phenyl)-N'-[2-(2,3-dichloro-phenylamino)-ethyl]-ethane-1,2-diamine C16H17Cl4N3
N-(2,3-Dichloro-phenyl)-N'-[2-(2,3-dichloro-phenylamino)-ethyl]-ethane-1,2-diamine N-(2,3-Dichloro-phenyl)-N'-[2-(2,3-dichloro-phenylamino)-ethyl]-ethane-1,2-diamine C16H17Cl4N3
Benzoic acid, 2-[(methylamino)sulfonyl]- Benzoic acid, 2-[(methylamino)sulfonyl]- 125372-22-7 C8H9NO4S
Benzene,1-ethoxy-4-(phenylmethyl)- Benzene,1-ethoxy-4-(phenylmethyl)- 35672-52-7 C15H16O
Donepezil  Impurity Donepezil Impurity 36159-03-2 C22H22O5
tert-Butyl 4-(4-nitropyridin-3-yl)piperazine-1-carboxylate tert-Butyl 4-(4-nitropyridin-3-yl)piperazine-1-carboxylate 1774898-55-3 C14H20N4O4
4-(2-Aminophenoxy)-N-methylpicolinamide 4-(2-Aminophenoxy)-N-methylpicolinamide 1153328-25-6 C13H13N3O2
Exemestane impurity B -F Exemestane impurity B -F 184972-09-6 C20H26O3
Fesoterodine Impurity 8 Fesoterodine Impurity 8 1380491-71-3 C48H66N2O4
QBWNUEJPRUVNTD-FOIQADDNSA-N QBWNUEJPRUVNTD-FOIQADDNSA-N 1346602-17-2 C22H17N3O5
JZWUWLJCMMPASK-YTSMVRMISA-N JZWUWLJCMMPASK-YTSMVRMISA-N 147103-95-5 C24H24N4O7S
KXEITTOLAUJQPU-UHFFFAOYSA-N KXEITTOLAUJQPU-UHFFFAOYSA-N 1797832-43-9 C20H21N7O2S
GPZGXSFHPLUMHE-KEUNHQLVSA-N GPZGXSFHPLUMHE-KEUNHQLVSA-N 157054-84-7 C52H81NO13
IRLGOHSHDYCIAB-ZMVBZRCWSA-N IRLGOHSHDYCIAB-ZMVBZRCWSA-N 1076198-14-5 C19H23F3N2O5
AOEGVETYSHPGBP-XYIDDFOTSA-N AOEGVETYSHPGBP-XYIDDFOTSA-N 1887057-05-7 C41H55NO14
4,6-DIAMINOINDAZOLE 4,6-DIAMINOINDAZOLE 885518-52-5 C7H8N4
12-Deoxy Roxithromycin 12-Deoxy Roxithromycin 425365-65-7 C41H76N2O14
1-(1-oxidopyridin-1-ium-2-yl)-1-phenylethanol 1-(1-oxidopyridin-1-ium-2-yl)-1-phenylethanol 100393-43-9 C13H13NO2
(R)-(R)-1-methylpyrrolidin-3-yl 2-cyclopentyl-2-hydroxy-2-phenylacetate(WXC03446) (R)-(R)-1-methylpyrrolidin-3-yl 2-cyclopentyl-2-hydroxy-2-phenylacetate(WXC03446) 616866-21-8 C18H25NO3
(R)-2-((6-(3-aminopiperidin-1-yl)-3-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)benzoic acid (R)-2-((6-(3-aminopiperidin-1-yl)-3-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)benzoic acid C18H22N4O4
(R)-2-((6-(3-aminopiperidin-1-yl)-3-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)benzoic acid (R)-2-((6-(3-aminopiperidin-1-yl)-3-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)methyl)benzoic acid C18H22N4O4
6'-methyl-3-(4-(methylsulfonyl)phenyl)-[2,3'-bipyridin]-5-amine 6'-methyl-3-(4-(methylsulfonyl)phenyl)-[2,3'-bipyridin]-5-amine C18H17N3O2S
Diquafosol Impurity 4 Diquafosol Impurity 4
Tofacitinib Impurity X Tofacitinib Impurity X
3-pentylideneisobenzofuran-1(3H)-one 3-pentylideneisobenzofuran-1(3H)-one 90965-68-7 C13H14O2
Solifenacin Related Compound 29 Solifenacin Related Compound 29 861998-77-8 C23H26N2O3
Ticagrelor Impurity 31 Ticagrelor Impurity 31
Ticagrelor Related Compound 46 Ticagrelor Related Compound 46 2205903-73-5 C28H42ClN9O8S2
Ticagrelor Related Compound 71 Ticagrelor Related Compound 71 1548397-10-9 C7H8ClN3O3S
Ticagrelor Related Compound 76 Ticagrelor Related Compound 76
Vonoprazan Impurity 25 Vonoprazan Impurity 25
1-(3,4-Dimethoxyphenyl)-2-(methylsulfonyl)ethan-1-amine 1-(3,4-Dimethoxyphenyl)-2-(methylsulfonyl)ethan-1-amine 1255909-25-1 C11H17NO4S
Adefovir Impurity C Adefovir Impurity C
Blonanserin Impurity M Blonanserin Impurity M
Brexpiprazole Impurity H Brexpiprazole Impurity H
Cefaclor EP Impurity H Cefaclor EP Impurity H
Flupentixol Impurity B Flupentixol Impurity B
Ipratropium Bromide Impurity J Ipratropium Bromide Impurity J C20H29Br2NO3
Cyclosporine iMpurity Cyclosporine iMpurity
ThiaMine IMpurity ThiaMine IMpurity C12H17ClN4OS
CitalopraM IMpurity CitalopraM IMpurity
EMpagliflozin  iMpurity 10 EMpagliflozin iMpurity 10 2069942-68-1 C32H37ClO12
EMpagliflozin  iMpurity 15 EMpagliflozin iMpurity 15
(S)-8-(3-aMinopiperidin-1-yl)-7-(but-2-ynyl)-3-Methyl-1-((4-Methylquinazolin-2-yl)Methyl)-1H-purine-2,6(3H,7H)-dione (S)-8-(3-aMinopiperidin-1-yl)-7-(but-2-ynyl)-3-Methyl-1-((4-Methylquinazolin-2-yl)Methyl)-1H-purine-2,6(3H,7H)-dione 668269-51-0 C25H28N8O2
ibrutinib iMpurity B ibrutinib iMpurity B
Sunitinib iMpurity A Sunitinib iMpurity A
Sunitinib iMpurity F Sunitinib iMpurity F
(3R,4S)-1-(3-fluorophenyl)-3-((S)-3-(4-fluorophenyl)-3-hydroxypropyl)-4-(4-hydroxyphenyl)azetidin-2-one (3R,4S)-1-(3-fluorophenyl)-3-((S)-3-(4-fluorophenyl)-3-hydroxypropyl)-4-(4-hydroxyphenyl)azetidin-2-one 1700622-06-5 C24H21F2NO3
Betamethasone EP Impurity J Betamethasone EP Impurity J 18383-24-9 C22H30O4
Cefazedone Related Impurity 10 Cefazedone Related Impurity 10 18884-65-6 C10H12N2O5S
Etoricoxib Impurity 8 Etoricoxib Impurity 8 1421227-97-5 C22H20N2O4S
Mupirocin Impurity E Mupirocin Impurity E C26H44O9
Phenylephrine Impurity 1 Phenylephrine Impurity 1 C9H13NO2
Rupatadine Impurity B Rupatadine Impurity B 156523-04-5 C26H24ClN3O
Theophylline Impurity 1 Theophylline Impurity 1 5614-53-9 C9H10N4O3
Venlafaxine N-Dimer Venlafaxine N-Dimer 1187545-61-4 C32H48N2O4
Chrysin 6-C-glucoside 8-C-arabinoside Chrysin 6-C-glucoside 8-C-arabinoside 185145-34-0 C26H28O13
ent-Ivabradine Hydrochloride ent-Ivabradine Hydrochloride 148849-68-7 C27H37ClN2O5
Sofosbuvir metabolites GS566500 Sofosbuvir metabolites GS566500 1233335-78-8 C13H19FN3O9P
Ondansetron impurity G (PhEur) Ondansetron impurity G (PhEur) 99614-04-7 C17H18ClN3O
4-Hydroxy-1-methyl-7-phenoxy-3-isoquinolinecarboxylic acid methyl ester 4-Hydroxy-1-methyl-7-phenoxy-3-isoquinolinecarboxylic acid methyl ester 1421312-34-6 C18H15NO4
2-[(4-METHYLPHENOXY)METHYL]BENZOIC ACID 2-[(4-METHYLPHENOXY)METHYL]BENZOIC ACID 728-97-2 C15H14O3
2-AMINO-3,5-DIBROMOBENZENE-1-CARBOHYDRAZIDE 2-AMINO-3,5-DIBROMOBENZENE-1-CARBOHYDRAZIDE 97096-13-4 C7H7Br2N3O
Trityl losartan Trityl losartan 133909-99-6 C41H37ClN6O
1-Piperazineethanol,4-[(2-chlorophenyl)(3-chlorophenyl)methyl]-, hydrochloride (1:2) 1-Piperazineethanol,4-[(2-chlorophenyl)(3-chlorophenyl)methyl]-, hydrochloride (1:2) 126517-38-2 C19H24Cl4N2O
2-chloromethyl-4-(3-hydroxypropoxy)-3-methylpyridine 2-chloromethyl-4-(3-hydroxypropoxy)-3-methylpyridine 117976-92-8 C10H14ClNO2
8-Methoxy Entecavir 8-Methoxy Entecavir 2203740-23-0 C13H17N5O4
Bortezomib Impurity 32 Bortezomib Impurity 32 1310383-72-2 C17H31BF3NO5
Topiroxostat Impurity 14 Topiroxostat Impurity 14
1-Pyrrolidinecarboxylic acid, 4-hydroxy-2-(3-thiazolidinylcarbonyl)-, 1,1-dimethylethyl ester, (2S,4S)- 1-Pyrrolidinecarboxylic acid, 4-hydroxy-2-(3-thiazolidinylcarbonyl)-, 1,1-dimethylethyl ester, (2S,4S)- 401564-34-9 C13H22N2O4S
Ampicillin Impurity B Ampicillin Impurity B
Clindamycin Phosphate EP Impurity K Clindamycin Phosphate EP Impurity K
UGDFSHGFPJSALM-OCKHKDLRSA-N UGDFSHGFPJSALM-OCKHKDLRSA-N 113806-01-2 C22H25NO3
Homoeriodictyol 7-O-glucoside Homoeriodictyol 7-O-glucoside 14982-11-7 C22H24O11
7,7'-(butane-1,4-diylbis(oxy))bis(quinolin-2(1H)-one) 7,7'-(butane-1,4-diylbis(oxy))bis(quinolin-2(1H)-one) 2116542-19-7 C22H20N2O4
Descyclopropyl Lenvatinib Descyclopropyl Lenvatinib 417719-51-8 C18H15ClN4O4
3-Piperidinemethanol, 4-(4-fluorophenyl)-1-methyl-, (3R,4R)-rel- 3-Piperidinemethanol, 4-(4-fluorophenyl)-1-methyl-, (3R,4R)-rel- 54088-21-0 C13H18FNO
1'-Hydroxy Bilastine 1'-Hydroxy Bilastine 1638785-23-5 C28H37N3O4
Brexpiprazole Impurity 4 Brexpiprazole Impurity 4 2116542-20-0 C25H27N3O2S
Fosaprepitant Impurity 5 Fosaprepitant Impurity 5 327623-35-8 C21H19F6NO3
Moxifloxacin Ethyl Ester Moxifloxacin Ethyl Ester 1403836-23-6 C23H28FN3O4
Pantoprazole Impurity 5 Pantoprazole Impurity 5 1806469-15-7 C8H6F2N2O2
Venlafaxine Impurity 5 Venlafaxine Impurity 5 1346600-77-8 C17H27NO2
N-(4-methyl-3-(4-methyl-6-(pyridin-3-yl)pyrimidin-2-ylamino)phenyl)-4-((4-methylpiperazin-1-yl)methyl)benzamide N-(4-methyl-3-(4-methyl-6-(pyridin-3-yl)pyrimidin-2-ylamino)phenyl)-4-((4-methylpiperazin-1-yl)methyl)benzamide C30H33N7O
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