同用名/別名（Synonym）
Leukemia Inhibitory Factor; LIF; Differentiation-Stimulating Factor; D Factor; Melanoma-Derived LPL Inhibitor; MLPLI; Emfilermin; LIF; HILDA
描述（Description）
LIF Protein, Mouse is a lymphoid factor involved in various neural and inflammatory processes such as the acute-phase reaction, tissue damage, and infection.
純度（Purity）
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
生物活性（Bioactivity）
1.The ED50 is <0.01 ng/mL as measured by M1 cells, corresponding to a specific activity of >1.0 × 108 units/mg.
2.Measured by its ability to induce IL-6 secretion by M-NFS-60 mouse cells. The ED50 for this effect is 2.407 ng/mL, corresponding to a specific activity is 4.15×105 U/mg.
3.Measured in a cell proliferation assay using C2C12 cell. The ED50 for this effect is 0.3801-0.412 ng/mL.
氨基酸序列（Target Protein Sequence）
SPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPFPNNVEKLCAPNMTDFPSFHGNGTEKTKLVELYRMVAYLSASLTNITRDQKVLNPTAVSLQVKLNATIDVMRGLLSNVLCRLCNKYRVGHVDVPPVPDHSDKEAFQRKKLGCQLLGTYKQVISVVVQAFSPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPF
制劑（Trait）
Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH 8.0 or 20mM PB, 150mM NaCl, pH 7.4 or 50 mM Tris-HCL, 300 mM NaCL, pH 8.0 or PBS, pH 7.4, 8% trehalose.
復(fù)溶（Reconstitution）
It is recommended to redissolve in sterile deionized water.
保存條件&效期（Storage & Stability）
Stored at -20°C for 1 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.Please avoid repeated freeze-thaw cycles.
運輸方式(Shipping)
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
生物活性（Bioactivity）
Measured in the M1 cell differentiation assay. The ED50 is ≤0.2ng/ml.
電泳（SDS-PAGE）
Recombinant Mouse LIF SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Fd004810 SDS-PAGE.
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關(guān)鍵字： GMP級;高純度;批間一致;生物活性;科研專供;
在生物科技領(lǐng)域，豐度蛋白專注重組蛋白供應(yīng)，以“豐度的風(fēng)帆為蛋白提供動力，豐度為蛋白護航”為使命，賦能生命科學(xué)發(fā)展。
蘇州豐度依托先進技術(shù)平臺，打造成熟生產(chǎn)體系，嚴控基因工程設(shè)計、細胞培養(yǎng)、蛋白純化等環(huán)節(jié)，優(yōu)化工藝與管理，確保產(chǎn)品高純度、高活性且批間一致。產(chǎn)品覆蓋基礎(chǔ)研究、生物醫(yī)藥研發(fā)等多領(lǐng)域，為科研及生產(chǎn)提供多樣、適配產(chǎn)品，助力突破技術(shù)難題 。