同用名/別名(Synonym)
Leukemia Inhibitory Factor; LIF; Differentiation-Stimulating Factor; D Factor; Melanoma-Derived LPL Inhibitor; MLPLI; Emfilermin; LIF; HILDA
描述(Description)
LIF Protein, Human is a lymphoid factor involved in various neural and inflammatory processes such as the acute-phase reaction, tissue damage, and infection.
純度(Purity)
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
生物活性(Bioactivity)
1.The ED50 is <0.2 ng/mL as measured by TF-1 cells, corresponding to a specific activity of >4.9 × 106 units/mg.
2.Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 25-204.1 pg/mL.
氨基酸序列(Target Protein Sequence)
SPLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGEPFPNNLDKLCGPNVTDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADILRGLLSNVLCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQAF
制劑(Trait)
Lyophilized after extensive dialysis against PBS
復(fù)溶(Reconstitution)
It is recommended to redissolve in sterile deionized water.
保存條件&效期(Storage & Stability)
Stored at -20°C for 1 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.Please avoid repeated freeze-thaw cycles.
運輸方式(Shipping)
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

生物活性(Bioactivity)
Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/m

電泳(SDS-PAGE)
Recombinant Human LIF SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with F1d004710 SDS-PAGE.
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關(guān)鍵字: GMP級;高純度;批間一致;生物活性;科研專供;
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