Biotinylated Human FAP Protein, His,Avitag?, active dimer (MALS verified)
分子別名（Synonym）
FAP,FAPalpha,SIMP,Seprase,APCE
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）
Biotinylated Human FAP Protein, His,Avitag (FAP-H82Q6) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asp 760 (Accession # Q12884-1).
Predicted N-terminus: His | His
蛋白結(jié)構(gòu)（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag?).
The protein has a calculated MW of 93.1 kDa. The protein migrates as 100-115 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
標(biāo)記（Labeling）
Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白標(biāo)記度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
純度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景介紹
成纖維細(xì)胞活化蛋白（FAP，亦稱seprase）是一種II型跨膜絲氨酸蛋白酶。其細(xì)胞膜結(jié)合型與可溶型均具有脯氨酸后切割內(nèi)肽酶活性，尤其偏好作用于Ala/Ser-Gly-Pro-Ser/Asn/Ala共有序列，同時可降解明膠和熱變性I型膠原。該蛋白酶還表現(xiàn)出二肽基肽酶活性，優(yōu)先水解Ala-Pro、Ile-Pro、Gly-Pro、Arg-Pro及Pro-Pro肽鍵。細(xì)胞膜結(jié)合型FAP通過與DPP4、PLAUR或整合素協(xié)同作用，參與細(xì)胞外基質(zhì)（ECM）的胞周蛋白水解過程，進而促進細(xì)胞在ECM中的黏附、遷移和侵襲。在腦實質(zhì)中，FAP通過降解蛋白聚糖brevican促進膠質(zhì)瘤細(xì)胞侵襲。在黑色素細(xì)胞中，該蛋白通過不依賴絲氨酸蛋白酶活性的方式調(diào)控細(xì)胞增殖與存活，發(fā)揮腫瘤抑制作用。
關(guān)鍵字： FAP;FAP蛋白;FAP重組蛋白;ACRO;百普賽斯;
百普賽斯集團ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團業(yè)務(wù)遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務(wù)客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。