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     Western blot analysis of Phospho-Cadherin 5 (Tyr731) in HepG2 lysates using Phospho-Cadherin 5 (Tyr731) antibody.    

  
  

Phospho-Cadherin 5 (Tyr731) antibody is a specialized tool used to detect the phosphorylated form of Cadherin 5 (also known as VE-cadherin or CDH5) at tyrosine residue 731. Cadherin 5 is a calcium-dependent cell-cell adhesion protein predominantly expressed in endothelial cells, where it plays a critical role in maintaining vascular integrity, regulating endothelial barrier function, and mediating angiogenesis. Phosphorylation at specific tyrosine residues, such as Tyr731. modulates Cadherin 5’s interactions with cytoplasmic partners like β-catenin and p120-catenin, influencing cell adhesion dynamics and intracellular signaling pathways.

This phosphorylation event is often associated with vascular endothelial growth factor (VEGF) signaling or inflammatory stimuli, which promote tyrosine kinase activation (e.g., Src family kinases). The Tyr731 site, in particular, has been implicated in regulating endothelial permeability and junctional stability. Phospho-Cadherin 5 (Tyr731) antibody enables researchers to study these mechanisms in contexts such as vascular development, tumor angiogenesis, or inflammatory diseases. It is commonly used in techniques like Western blotting, immunofluorescence, or immunohistochemistry to assess phosphorylation-dependent changes in Cadherin 5 localization or function.

Validation of this antibody typically includes testing in endothelial cell models or tissues under conditions that induce tyrosine phosphorylation, with specificity confirmed via knockout controls or phosphatase treatment. Its applications span basic research in vascular biology and translational studies targeting pathological angiogenesis or vascular leakage.