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     Western blot analysis of Phospho-PAK4 + PAK5 + PAK6 (Ser474 + Ser560 + Ser602) in Hela, A549, HL-60, U2OS, U87-MG lysates using Phospho-PAK4/5/6 (Ser474/Ser560/Ser602) antibody.    

  
  

The Phospho-PAK4/5/6 (Ser474/Ser560/Ser602) antibody is designed to detect the activated forms of PAK4. PAK5. and PAK6. members of the p21-activated kinase (PAK) family. These serine/threonine kinases play critical roles in regulating cytoskeletal dynamics, cell proliferation, survival, and migration. PAK4. PAK5 (also known as PAK7), and PAK6 belong to Group II PAKs, which are activated by Rho-family GTPases like Cdc42 and Rac. Their activation involves phosphorylation at specific residues within their kinase domains: Ser474 in PAK4. Ser560 in PAK5. and Ser602 in PAK6. These phosphorylation events are essential for their catalytic activity and downstream signaling.

This antibody is widely used in research to study the activation status of Group II PAKs in cellular pathways, particularly in cancer, where PAK overexpression or hyperactivation is linked to tumor progression, invasion, and resistance to therapy. It is also employed in neuroscience, as PAK5/PAK6 are implicated in neuronal development and neurodegenerative diseases. Specificity for the phosphorylated forms allows researchers to distinguish active kinases from their inactive counterparts in techniques like Western blotting, immunofluorescence, or immunohistochemistry. Validation typically includes testing in knockout models or phosphorylation-deficient mutants to ensure accurate detection. Understanding PAK4/5/6 activation provides insights into cell signaling mechanisms and potential therapeutic targets.