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     Western blot analysis of MethylHistone H3 (di K4) in HeLa lysates using DiMethyl-Histone H3 (Lys4) antibody.    

  
  

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     Immunohistochemistry analysis of paraffin-embedded Human liver using MethylHistone H3 (di K4) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    

  
  

The dimethylated form of histone H3 at lysine 4 (H3K4me2) is a key epigenetic modification associated with actively transcribed gene promoters and regulatory elements. Histone H3. one of the core components of nucleosomes, undergoes post-translational modifications that regulate chromatin structure and gene expression. Methylation at lysine 4 (K4) of histone H3 is catalyzed by histone methyltransferases, such as the COMPASS family or MLL complexes, and its levels (mono-, di-, or tri-methylation) correlate with distinct transcriptional states. H3K4me2. specifically, marks regions of poised or moderately active transcription, often overlapping with enhancers and promoter-proximal regions. Unlike H3K4me3 (linked to highly active promoters) or H3K4me1 (enriched at enhancers), H3K4me2 serves as a dynamic intermediate in transcriptional activation or repression, depending on cellular context and interacting partners.

Antibodies targeting H3K4me2 are critical tools in chromatin immunoprecipitation (ChIP) assays, immunoblotting, and immunofluorescence to map its genomic distribution and study its role in development, differentiation, and disease. Dysregulation of H3K4 methylation is implicated in cancers, neurological disorders, and developmental defects, making these antibodies valuable for both basic research and clinical diagnostics. Specificity validation via knockout controls or peptide competition is essential, as cross-reactivity with similar methylated residues (e.g., H3K9me2) can occur. Overall, H3K4me2 antibodies provide insights into epigenetic mechanisms governing gene regulation and cellular identity.