價(jià)格 | 詢價(jià) | ||
包裝 | 20μl | 50μl | 100μl |
最小起訂量 | 1μl |
發(fā)貨地 | 上海 |
更新日期 | 2025-06-03 |
中文名稱:DiMethyl-Histone H3 (Lys27)抗體 | 英文名稱:Mouse Monoclonal DiMethyl-HistoneH3(Lys27) Antibody |
品牌: 艾普蒂 | 產(chǎn)地: 武漢 |
保存條件: Stored at -20°C for 8914 year. Avoid repeated freeze / thaw cycles. | 產(chǎn)品類別: 抗體 |
重組: 否 | 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA |
種屬反應(yīng)性: Human,Mouse,Rat | 宿主: Mouse |
偶聯(lián)物: 無 | 靶點(diǎn): DiMethyl-Histone H3 (Lys27) |
WB | 咨詢技術(shù) | Human,Mouse,Rat |
IF | 1/20 | Human,Mouse,Rat |
IHC | 咨詢技術(shù) | Human,Mouse,Rat |
ICC | 1/50-1/200 | Human,Mouse,Rat |
FCM | 咨詢技術(shù) | Human,Mouse,Rat |
Elisa | 咨詢技術(shù) | Human,Mouse,Rat |
Aliases | H3K27me2; H3 histone; HIST1H3A; Histone cluster 1; H3a |
Entrez GeneID | 8350 |
clone | 5H6 |
WB Predicted band size | Calculated MW: 15 kDa; Observed MW: 15 kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Rat |
Immunogen | Synthetic Peptide of Histone H3 (Di Methyl Lys27) |
Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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Western blot analysis of DiMethyl-Histone H3 (Lys27) in Hela, rat heart, mouse heart, HUVEC, Jurkat lysates using DiMethyl-Histone H3 (Lys27) antibody.
Western blot analysis of DiMethyl-Histone H3 (Lys27) in C6, 3T3, C2C12 lysates using DiMethyl-Histone H3 (Lys27) antibody
Immunoprecipitation analysis of DiMethyl-Histone H3 (Lys27) in Hela lysates using DiMethyl-Histone H3 (Lys27) antibody.
Immunocytochemistry analysis ofDiMethyl-Histone H3 (Lys27) in HeLa cells using DiMethyl-Histone H3 (Lys27) antibody.
以下是3篇關(guān)于H3K27me2(Dimethyl-Histone H3 Lys27)抗體的經(jīng)典文獻(xiàn)摘要,供參考:
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1. **文獻(xiàn)名稱**: *Histone H3K27 trimethylation and H3K4 methylation define different chromatin states in Polycomb silencing*
**作者**: Boyer LA et al.
**摘要**: 該研究通過ChIP-seq技術(shù),使用H3K27me2/me3抗體揭示Polycomb復(fù)合物(PRC)介導(dǎo)的基因抑制機(jī)制,發(fā)現(xiàn)H3K27二甲基化與三甲基化在胚胎干細(xì)胞分化中具有不同的表觀遺傳調(diào)控功能。
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2. **文獻(xiàn)名稱**: *Role of histone H3 lysine 27 methylation in Polycomb-group silencing*
**作者**: Cao R et al.
**摘要**: 本文利用H3K27me2特異性抗體進(jìn)行染色質(zhì)免疫沉淀實(shí)驗(yàn),證明EZH2(PRC2核心催化亞基)介導(dǎo)的H3K27二甲基化和三甲基化是維持干細(xì)胞多能性和基因沉默的關(guān)鍵表觀遺傳標(biāo)記。
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3. **文獻(xiàn)名稱**: *A histone H3 lysine 27-specific antibody validates Drosophila Polycomb function*
**作者**: Pengelly AR et al.
**摘要**: 作者開發(fā)并驗(yàn)證了H3K27me2抗體的特異性,通過果蠅模型證明H3K27二甲基化在發(fā)育基因調(diào)控中與Polycomb抑制復(fù)合物協(xié)同作用,且其分布模式與三甲基化存在差異。
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4. **文獻(xiàn)名稱**: *Histone modifications: from genome-wide maps to functional insights*
**作者**: Bernstein BE et al.
**摘要**: 該綜述系統(tǒng)總結(jié)了H3K27me2等組蛋白修飾的檢測方法(如抗體特異性驗(yàn)證),并探討其在染色質(zhì)結(jié)構(gòu)動(dòng)態(tài)調(diào)控及癌癥等疾病中的潛在作用機(jī)制。
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提示:具體實(shí)驗(yàn)引用時(shí)建議通過PubMed或Google Scholar核對最新文獻(xiàn),關(guān)注抗體驗(yàn)證方法(如Western blot/ChIP的示例數(shù)據(jù))以確保可靠性。
The DiMethyl-Histone H3 (Lys27) antibody specifically detects histone H3 proteins dimethylated at lysine 27 (H3K27me2), a post-translational modification critical for epigenetic regulation. Histone H3 is a core component of nucleosomes, and lysine methylation modulates chromatin structure and gene expression. H3K27me2 is associated with transcriptional repression and serves as a dynamic marker of chromatin states. Unlike the well-characterized H3K27me3 (trimethylation), which is strongly linked to Polycomb Repressive Complex 2 (PRC2)-mediated gene silencing in development and cancer, H3K27me2 is more broadly distributed and may play roles in maintaining facultative heterochromatin or priming regions for PRC2 activity.
This antibody is widely used in chromatin immunoprecipitation (ChIP), immunofluorescence (IF), and Western blotting to study epigenetic mechanisms in cellular differentiation, oncogenesis, and stem cell biology. Aberrant H3K27 methylation is implicated in diseases, including malignancies like gliomas and lymphomas, where altered PRC2 activity disrupts normal methylation patterns. Researchers rely on the specificity of H3K27me2 antibodies to distinguish between methylation states, often validating results with demethylase treatments or knockouts. Cross-reactivity with H3K27me1/me3 must be ruled out to ensure accurate interpretation. Understanding H3K27me2 dynamics provides insights into how cells balance gene activation and repression during development and disease progression.
成立日期 | 2024-07-02 (2年) | 注冊資本 | 20萬人民幣 |
員工人數(shù) | 1-10人 | 年?duì)I業(yè)額 | ¥ 100萬以內(nèi) |
主營行業(yè) | 抗體,植物生物技術(shù),細(xì)胞培養(yǎng),蛋白組學(xué) | 經(jīng)營模式 | 貿(mào)易,工廠,試劑,定制,服務(wù) |
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