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Acetyl-Histone H3 (Lys27)抗體,Rabbit Monoclonal Acetyl-HistoneH3(Lys27) Antibody
  • Acetyl-Histone H3 (Lys27)抗體,Rabbit Monoclonal Acetyl-HistoneH3(Lys27) Antibody
  • Acetyl-Histone H3 (Lys27)抗體,Rabbit Monoclonal Acetyl-HistoneH3(Lys27) Antibody
  • Acetyl-Histone H3 (Lys27)抗體,Rabbit Monoclonal Acetyl-HistoneH3(Lys27) Antibody

Acetyl-Histone H3 (Lys27)抗體;Acetyl-Histone H3 (Lys27) Antibody—艾普蒂

價格 詢價
包裝 20μl 50μl 100μl
最小起訂量 1μl
發(fā)貨地 上海
更新日期 2025-06-03
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產(chǎn)品詳情

中文名稱:Acetyl-Histone H3 (Lys27)抗體英文名稱:Rabbit Monoclonal Acetyl-HistoneH3(Lys27) Antibody
品牌: 艾普蒂產(chǎn)地: 武漢
保存條件: Stored at -20°C for 8896 year. Avoid repeated freeze / thaw cycles.產(chǎn)品類別: 抗體
重組: 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA
種屬反應(yīng)性: Human,Mouse,Rat 宿主: Rabbit
偶聯(lián)物: 靶點: Acetyl-Histone H3 (Lys27)
2025-06-03 Acetyl-Histone H3 (Lys27)抗體 Rabbit Monoclonal Acetyl-HistoneH3(Lys27) Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武漢 Stored at -20°C for 8896 year. Avoid repeated freeze / thaw cycles. 抗體

驗證與應(yīng)用

應(yīng)用及物種
WB咨詢技術(shù) Human,Mouse,Rat
IF1/20 Human,Mouse,Rat
IHC1/50-1/100 Human,Mouse,Rat
ICC1/50-1/200 Human,Mouse,Rat
FCM咨詢技術(shù) Human,Mouse,Rat
Elisa咨詢技術(shù) Human,Mouse,Rat
   

產(chǎn)品詳情

AliasesH3K27ac; H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J
Entrez GeneID8350
WB Predicted band sizeCalculated MW: 15 kDa; Observed MW: 15 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthetic acetyl-peptide corresponding to residues surrounding Lys27 of human Histone H3
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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       Western blot analysis of Histone H3 (acetylLys27) in Hela, A549, HL-60 lysates using Acetyl-Histone H3 (Lys27) antibody.    


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       Immunocytochemistry analysis of Histone H3 (acetylLys27) (green) in hela using Histone H3 (acetylLys27) antibody,and DAPI(blue)    


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       Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Histone H3 (acetylLys27) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    


           

參考文獻(xiàn)

以下是關(guān)于Acetyl-Histone H3 (Lys27)抗體的3篇參考文獻(xiàn),按文獻(xiàn)名稱、作者和摘要內(nèi)容分類整理:

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1. **文獻(xiàn)名稱**: "Histone H3K27ac separates active from poised enhancers and predicts developmental state"

**作者**: Creyghton, M.P. 等

**摘要**: 該研究利用H3K27ac抗體通過ChIP-seq技術(shù),區(qū)分了活性增強子與靜息態(tài)增強子,發(fā)現(xiàn)H3K27ac標(biāo)記與細(xì)胞發(fā)育階段密切相關(guān),為表觀遺傳調(diào)控提供了關(guān)鍵分子標(biāo)志。

2. **文獻(xiàn)名稱**: "Global mapping of H3K27ac in cancer reveals oncogenic reprogramming of enhancer landscapes"

**作者**: Zhang, X. 等

**摘要**: 研究通過H3K27ac抗體分析多種癌癥模型,揭示了腫瘤細(xì)胞中增強子區(qū)域乙酰化異常,表明H3K27ac在癌基因激活和腫瘤發(fā)生中起重要作用。

3. **文獻(xiàn)名稱**: "Dynamic acetylation of histone H3K27 marks developmental transitions in mammalian chromatin"

**作者**: Tie, F. 等

**摘要**: 該文獻(xiàn)使用H3K27ac抗體探究胚胎干細(xì)胞分化過程中組蛋白修飾的動態(tài)變化,發(fā)現(xiàn)其與關(guān)鍵發(fā)育基因的時序性激活直接相關(guān),為細(xì)胞命運決定提供機(jī)制解釋。

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這些研究均通過H3K27ac抗體揭示該修飾在基因調(diào)控、疾病機(jī)制及發(fā)育生物學(xué)中的功能,展示了該抗體在染色質(zhì)分析中的廣泛應(yīng)用。

       

背景信息

The Acetyl-Histone H3 (Lys27) antibody is a key tool for studying epigenetic regulation, specifically targeting the acetylation of lysine 27 on histone H3 (H3K27ac). Histone acetylation is a post-translational modification critical for chromatin structure and gene expression regulation. By neutralizing the positive charge of lysine residues, acetylation reduces histone-DNA interactions, promoting an open chromatin state that facilitates transcriptional activation. H3K27ac is enriched at active enhancers and promoters, serving as a hallmark of regulatory elements involved in cell-specific gene programs. Unlike H3K27 methylation (e.g., H3K27me3. associated with Polycomb-mediated gene silencing), H3K27ac is linked to transcriptional activation and is dynamically regulated by histone acetyltransferases (HATs) and deacetylases (HDACs).

This antibody is widely used in chromatin immunoprecipitation (ChIP) assays to map genomic regions associated with active transcription, as well as in techniques like Western blotting and immunofluorescence to assess acetylation levels in various biological contexts. Dysregulation of H3K27ac has been implicated in diseases such as cancer, where aberrant enhancer activity drives oncogene expression, and in neurological disorders. Researchers rely on validated Acetyl-Histone H3 (Lys27) antibodies to explore epigenetic mechanisms, evaluate drug effects targeting acetylation pathways, and identify biomarkers for disease diagnosis or therapy. Its specificity and reliability make it essential for studies linking chromatin dynamics to cellular function and disease.

       
關(guān)鍵字: Acetyl-Histone H3 (L;Acetyl-Histone H3 (L;Acetyl-Histone H3 (L;

公司簡介

生物技術(shù)有限公司
成立日期 2024-07-02 (2年) 注冊資本 20萬人民幣
員工人數(shù) 1-10人 年營業(yè)額 ¥ 100萬以內(nèi)
主營行業(yè) 抗體,植物生物技術(shù),細(xì)胞培養(yǎng),蛋白組學(xué) 經(jīng)營模式 貿(mào)易,工廠,試劑,定制,服務(wù)
  • 上海切爾齊生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注冊資本:20萬人民幣
  • 企業(yè)類型:有限責(zé)任公司(自然人投資或控股)
  • 主營產(chǎn)品:生物試劑
  • 公司地址:青浦
詢盤

Acetyl-Histone H3 (Lys27)抗體;Acetyl-Histone H3 (Lys27) Antibody—艾普蒂相關(guān)廠家報價

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廣州威佳科技有限公司
2025-08-02
詢價
上海點創(chuàng)生物科技有限公司
2025-08-02
¥1280
VIP3年
上海滬震實業(yè)有限公司
2025-08-01
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