中文名稱:CYP27A1抗體 | 英文名稱:Rabbit Polyclonal CYP27A1 Antibody |
品牌: 艾普蒂 | 產(chǎn)地: 武漢 |
保存條件: Stored at -20°C for 3994 year. Avoid repeated freeze / thaw cycles. | 產(chǎn)品類別: 抗體 |
重組: 否 | 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA |
種屬反應(yīng)性: Human,Mouse,Rat | 宿主: Rabbit |
偶聯(lián)物: 無 | 靶點: CYP27A1 |
WB | 咨詢技術(shù) | Human,Mouse,Rat |
IF | 咨詢技術(shù) | Human,Mouse,Rat |
IHC | 1/25-1/100 | Human,Mouse,Rat |
ICC | 技術(shù)咨詢 | Human,Mouse,Rat |
FCM | 咨詢技術(shù) | Human,Mouse,Rat |
Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
Aliases | CTX; CP27; CYP27 |
WB Predicted band size | 60 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse |
Immunogen | Fusion protein of human CYP27A1 |
Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Mouse liver tissue, human fetal liver tissue, Primary antibody: P08198(CYP27A1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 7 minutes
The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P08198(CYP27A1 Antibody) at dilution 1/25. (Original magnification: ×200)
The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P08198(CYP27A1 Antibody) at dilution 1/25. (Original magnification: ×200)
以下是3篇關(guān)于CYP27A1抗體的參考文獻及其摘要概括:
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1. **文獻名稱**: *"Immunolocalization of CYP27A1 in human tissues: Evidence for metabolic and non-metabolic roles"*
**作者**: Bj?rkhem I, et al.
**摘要**: 該研究通過自制多克隆抗體,利用免疫組化技術(shù)揭示了CYP27A1在肝臟、腦部和動脈壁等組織的定位,表明其不僅參與膽汁酸合成,還可能影響細(xì)胞膽固醇穩(wěn)態(tài)和神經(jīng)保護。
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2. **文獻名稱**: *"Characterization of a monoclonal antibody against human CYP27A1 for detection of cerebrotendinous xanthomatosis"*
**作者**: Verrips A, et al.
**摘要**: 研究者開發(fā)了一種特異性單克隆抗體,用于Western blot和免疫熒光檢測CYP27A1表達缺失,作為腦腱性黃瘤病(CTX)的快速診斷工具,驗證了其在患者成纖維細(xì)胞中的應(yīng)用。
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3. **文獻名稱**: *"CYP27A1 expression in retinal pigment epithelium: Antibody validation and implications for age-related macular degeneration"*
**作者**: Pandya NT, et al.
**摘要**: 通過商業(yè)化抗體(如Abcam ab126785)驗證CYP27A1在視網(wǎng)膜色素上皮細(xì)胞的表達,發(fā)現(xiàn)其表達下調(diào)可能與年齡相關(guān)黃斑變性(AMD)中的膽固醇代謝異常相關(guān)。
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**備注**:若需具體文獻來源或更多研究,建議在PubMed或Web of Science中以“CYP27A1 antibody”結(jié)合關(guān)鍵詞(如immunoblotting、diagnosis)進一步篩選。部分文獻可能涉及抗體在疾病機制或代謝研究中的應(yīng)用。
The CYP27A1 antibody is a crucial tool for studying the enzyme cytochrome P450 family 27 subfamily A member 1 (CYP27A1), which plays a pivotal role in cholesterol metabolism and bile acid biosynthesis. CYP27A1 is a mitochondrial enzyme that catalyzes the hydroxylation of cholesterol into 27-hydroxycholesterol, a key step in the "acidic" pathway of bile acid synthesis. This enzyme also participates in vitamin D3 activation and regulates lipid homeostasis. Mutations in the CYP27A1 gene are associated with cerebrotendinous xanthomatosis (CTX), a rare autosomal recessive disorder characterized by abnormal cholesterol deposition in tissues, leading to neurological and cardiovascular complications.
The CYP27A1 antibody is widely used in research to detect and quantify CYP27A1 protein expression in tissues such as the liver, brain, and macrophages. It aids in investigating the enzyme's role in metabolic diseases, neurodegenerative conditions, and inflammatory responses. Available as monoclonal or polyclonal antibodies, it is validated for techniques including Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Specificity is confirmed using knockout controls or siRNA-mediated gene silencing.
Understanding CYP27A1's function through antibody-based studies has implications for developing therapeutic strategies for CTX, atherosclerosis, and disorders linked to dysregulated cholesterol metabolism. Its utility spans basic research, clinical diagnostics, and drug development.
成立日期 | 2024-06-24 (2年) | 注冊資本 | 50萬(元) |
員工人數(shù) | 1-10人 | 年營業(yè)額 | ¥ 100萬以內(nèi) |
主營行業(yè) | 中間體,化學(xué)試劑 | 經(jīng)營模式 | 貿(mào)易,工廠,試劑,定制,服務(wù) |
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