The DOK5 antibody is a research tool designed to detect and analyze the DOK5 protein, a member of the "docking protein" (DOK) family. DOK proteins are cytoplasmic adaptors that mediate signal transduction downstream of receptor tyrosine kinases (RTKs) and cytokine receptors. DOK5. specifically, is prominently expressed in neuronal tissues, the pancreas, and skeletal muscle, where it regulates cellular processes like differentiation, survival, and glucose metabolism. Structurally, DOK5 contains an N-terminal pleckstrin homology (PH) domain, a central phosphotyrosine-binding (PTB) domain for interacting with phosphorylated receptors, and multiple C-terminal tyrosine residues that recruit downstream signaling molecules.
DOK5 is known to interact with RET, insulin receptors, and neurotrophic receptors (e.g., TrkB), modulating pathways such as Ras/MAPK and PI3K/Akt. Its role in neuronal development and metabolic homeostasis makes it a focus in studies on neurodegenerative diseases, diabetes, and cancer. Aberrant DOK5 expression or mutations have been linked to insulin resistance, neuroblastoma, and Parkinson’s disease. The DOK5 antibody is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression, localization, and post-translational modifications in experimental models. Researchers utilize this antibody to explore DOK5's mechanistic contributions to disease pathogenesis and its potential as a therapeutic target.