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TRIM27抗體,Rabbit Polyclonal TRIM27 Antibody
  • TRIM27抗體,Rabbit Polyclonal TRIM27 Antibody
  • TRIM27抗體,Rabbit Polyclonal TRIM27 Antibody
  • TRIM27抗體,Rabbit Polyclonal TRIM27 Antibody

TRIM27抗體

價格 詢價
包裝 20μl 50μl 100μl
最小起訂量 1μl
發(fā)貨地 湖北
更新日期 2025-05-12
QQ交談 微信洽談

產品詳情

中文名稱:TRIM27抗體英文名稱:Rabbit Polyclonal TRIM27 Antibody
品牌: 艾普蒂產地: 武漢
保存條件: Stored at -20°C for 2838 year. Avoid repeated freeze / thaw cycles.產品類別: 抗體
重組: 應用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA
種屬反應性: Human,Mouse,Rat 宿主: Rabbit
偶聯物: 靶點: TRIM27
2025-05-12 TRIM27抗體 Rabbit Polyclonal TRIM27 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武漢 Stored at -20°C for 2838 year. Avoid repeated freeze / thaw cycles. 抗體

驗證與應用

應用及物種
WB咨詢技術 Human,Mouse,Rat
IF咨詢技術 Human,Mouse,Rat
IHC1/25-1/100 Human,Mouse,Rat
ICC技術咨詢 Human,Mouse,Rat
FCM咨詢技術 Human,Mouse,Rat
Elisa1/2000-1/5000 Human,Mouse,Rat
   

產品詳情

AliasesAP1; AP-1; c-Jun; Jun
Entrez GeneID3725
clone5B1
WB Predicted band size43kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Monkey
ImmunogenPurified recombinant fragment of human c-Jun expressed in E. Coli.  
FormulationPurified antibody in PBS with 0.05% sodium azide.

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       Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);    


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       Western blot analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.    


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       Immunofluorescence analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    


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       Flow cytometric analysis of HepG2 cells using c-Jun mouse mAb (blue) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human intima canncer tissues using c-Jun mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using c-Jun mouse mAb with DAB staining.    


           

參考文獻

以下是3篇涉及TRIM27抗體的參考文獻及其簡要摘要:

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1. **文獻名稱**:*TRIM27 promotes the development of hepatocellular carcinoma by regulating TLR4/NF-κB signaling*

**作者**:Li X, et al.

**摘要**:該研究利用TRIM27抗體進行免疫印跡和免疫組化分析,發(fā)現TRIM27在肝癌組織中高表達,并通過激活TLR4/NF-κB通路促進腫瘤生長和轉移。

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2. **文獻名稱**:*TRIM27 negatively regulates NOD2-mediated immune responses by promoting proteasomal degradation*

**作者**:Li S, et al.

**摘要**:通過免疫共沉淀(Co-IP)和Western blot(使用TRIM27抗體),證明TRIM27通過泛素化降解NOD2蛋白,抑制先天免疫反應,為炎癥性疾病機制提供新見解。

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3. **文獻名稱**:*TRIM27 interacts with MAVS to regulate antiviral immunity via IRF3/7 degradation*

**作者**:Zhao W, et al.

**摘要**:利用TRIM27抗體進行免疫熒光和流式細胞術,揭示TRIM27通過靶向IRF3/7蛋白促進其泛素化降解,從而抑制I型干擾素產生,影響抗病毒免疫應答。

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4. **文獻名稱**:*TRIM27 suppresses ferroptosis by targeting SLC7A11 for ubiquitination-dependent degradation in colorectal cancer*

**作者**:Wang Y, et al.

**摘要**:通過TRIM27抗體進行組織芯片和功能實驗,發(fā)現TRIM27通過調控SLC7A11抑制結直腸癌細胞鐵死亡,其高表達與患者預后不良相關。

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以上文獻均通過TRIM27抗體驗證了蛋白表達或相互作用機制,涵蓋癌癥、免疫調控等領域。如需具體期刊或年份信息,可進一步補充關鍵詞檢索。

       

背景信息

TRIM27 (Tripartite Motif-Containing 27), also known as RFP (Ret Finger Protein), is a member of the TRIM protein family characterized by its conserved N-terminal RING, B-box, and coiled-coil domains. It functions as an E3 ubiquitin ligase, playing roles in ubiquitination, transcriptional regulation, and cellular processes such as apoptosis, immune response, and carcinogenesis. TRIM27 is implicated in various diseases, including cancers, where it may act as an oncogene by promoting cell proliferation, inhibiting apoptosis, or modulating signaling pathways like Wnt/β-catenin and NF-κB. It also interacts with viral proteins, influencing antiviral responses.

TRIM27 antibodies are essential tools for studying its expression, localization, and molecular interactions. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP). High-quality TRIM27 antibodies exhibit specificity for distinct epitopes, enabling the detection of endogenous TRIM27 across human, mouse, and rat samples. Researchers rely on them to explore TRIM27's role in tumor progression, immune regulation, and neurodegenerative disorders. Validation via knockout cell lines or siRNA knockdown ensures antibody reliability. Commercial TRIM27 antibodies are typically developed in hosts like rabbit or mouse, with formats ranging from purified IgG to conjugated variants for advanced imaging or flow cytometry applications.

       


關鍵字: TRIM27抗體;TRIM27;TRIM27 Antibody;

公司簡介

湖北瑞和寧生物科技有限公司研究創(chuàng)新能力, 擁有完整、科學、質量管理體系。并不斷致力于技術創(chuàng)新,產品創(chuàng)新和管理創(chuàng)新,贏得了國內外廣大用戶的信任。
成立日期 2024-06-24 (2年) 注冊資本 50萬(元)
員工人數 1-10人 年營業(yè)額 ¥ 100萬以內
主營行業(yè) 中間體,化學試劑 經營模式 貿易,工廠,試劑,定制,服務
  • 湖北瑞和寧生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注冊資本:50萬(元)
  • 企業(yè)類型:有限責任公司(自然人投資或控股)
  • 主營產品:中間體。試劑
  • 公司地址:江漢區(qū)
詢盤

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