RORA (Retinoic Acid Receptor-Related Orphan Receptor Alpha) is a nuclear hormone receptor that plays critical roles in regulating circadian rhythms, immune function, metabolism, and neuronal development. As a transcription factor, it binds to specific DNA response elements to activate or repress target genes, including those involved in inflammation, lipid homeostasis, and neurodevelopmental pathways. RORA dysfunction has been implicated in various diseases, such as autism spectrum disorder, bipolar disorder, cancer, and autoimmune conditions like multiple sclerosis.
RORA antibodies are essential tools for studying its expression, localization, and molecular interactions. They enable detection of RORA protein levels in tissues or cell lines via techniques like Western blotting, immunohistochemistry, and flow cytometry. Additionally, these antibodies facilitate chromatin immunoprecipitation (ChIP) to map RORA-binding genomic regions and elucidate its regulatory networks. Commercially available RORA antibodies are typically developed using immunogenic peptide sequences from conserved regions of the protein, ensuring specificity across human, mouse, and rat homologs. Validation steps, including knockout cell line controls, are crucial to confirm antibody reliability. Research utilizing RORA antibodies continues to advance understanding of its dual roles in health and disease, particularly its crosstalk with metabolic and inflammatory signaling pathways.