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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Human kidney tissue lysate, Primary antibody: P04918(GPR78 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    

  
  

The GPR78 antibody is a research tool designed to detect GPR78 (G Protein-Coupled Receptor 78), a member of the G protein-coupled receptor (GPCR) family. GPR78 is an orphan receptor, as its endogenous ligand and precise physiological functions remain poorly characterized. It shares structural homology with other GPCRs involved in cellular signaling, suggesting potential roles in modulating cAMP pathways, neurotransmission, or stress responses. Studies link GPR78 to neurological disorders, metabolic regulation, and cancer progression, though its mechanisms are under investigation.

The GPR78 antibody is typically developed in host species (e.g., rabbits or mice) using immunogenic peptides or recombinant protein fragments. It enables detection of GPR78 expression and localization via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Validation includes testing for specificity using knockout controls or siRNA-mediated knockdown. Researchers use this antibody to explore GPR78’s tissue distribution, overexpression in disease models, and interaction networks. Challenges include ensuring minimal cross-reactivity with related GPCRs and optimizing protocols for low-abundance targets.

As GPCRs are key therapeutic targets, GPR78 antibodies contribute to unraveling its pathophysiological relevance, aiding drug discovery and biomarker studies. Commercial availability from biotech vendors supports its broad application in basic and translational research.