The LSM11 antibody is a research tool designed to detect LSM11. a protein belonging to the Like Sm (LSM) family, which plays critical roles in RNA metabolism. LSM11 is a core component of the histone mRNA processing complex, specifically interacting with LSM10 to form a heterodimer essential for the 3'-end processing of replication-dependent histone pre-mRNAs. Unlike most eukaryotic mRNAs, histone mRNAs lack polyadenylation and instead form a conserved stem-loop structure. LSM11. along with other proteins like FLASH and U7 snRNP, facilitates this unique processing mechanism, which is tightly linked to cell cycle progression, particularly during S-phase.
LSM11 is also implicated in the assembly of the survival of motor neuron (SMN) complex, contributing to spliceosomal small nuclear ribonucleoprotein (snRNP) biogenesis. Dysregulation of LSM11 has been associated with developmental defects and diseases, including cancers and neurodegenerative disorders. Antibodies targeting LSM11 are widely used in techniques like immunoblotting, immunoprecipitation, and immunofluorescence to study its expression, localization, and interactions in cellular models. Researchers employ these antibodies to explore its role in histone mRNA processing, cell cycle regulation, and potential links to pathologies. Validation of LSM11 antibodies typically includes testing in knockout cell lines to confirm specificity, ensuring reliable detection across human, mouse, and other model organisms.