The KLHL2 (Kelch-like family member 2) antibody is a tool used to study the KLHL2 protein, a member of the Kelch-like family characterized by its BTB (Broad-Complex, Tramtrack, and Bric-à-brac) domain and Kelch repeat motifs. KLHL2 functions as a substrate-specific adaptor for Cullin3 (Cul3)-based E3 ubiquitin ligase complexes, facilitating the ubiquitination and subsequent degradation of target proteins. This protein is implicated in cellular processes such as cytoskeletal organization, ion channel regulation, and neuronal development. Its role in ubiquitination pathways links it to maintaining cellular homeostasis and stress responses.
KLHL2 antibodies are widely employed in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunohistochemistry, and immunoprecipitation. Studies have explored KLHL2's involvement in diseases, including cancers and neurological disorders, where dysregulation of ubiquitination may contribute to pathogenesis. For example, KLHL2 has been shown to regulate N-type voltage-gated calcium channels in neurons, influencing synaptic transmission.
Antibody specificity is validated using knockout controls or siRNA-mediated knockdowns to ensure reliable results. Commercially available KLHL2 antibodies are typically raised in rabbits or mice, with reactivity confirmed across human, mouse, and rat samples. Ongoing research aims to clarify its tissue-specific roles and therapeutic potential in ubiquitin-proteasome system-related pathologies.