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     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: 823 cells, Primary antibody: P01302(DTNB Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes    

  
  

The DTNB antibody is primarily associated with the detection of protein thiol groups modified by 5.5'-dithiobis-(2-nitrobenzoic acid) (DTNB), a widely used reagent in biochemistry for quantifying free sulfhydryl (-SH) groups. DTNB, also known as Ellman's reagent, reacts with thiols to form mixed disulfide bonds, releasing 2-nitro-5-thiobenzoic acid (TNB), which can be measured spectrophotometrically. Antibodies targeting DTNB-modified proteins are developed to specifically recognize TNB-conjugated residues, enabling the study of thiol redox states or protein conformational changes in biological systems.

These antibodies have applications in redox proteomics, where they help identify cysteine residues susceptible to oxidative modifications under stress conditions. They are also used in immunoassays (e.g., ELISA, Western blot) to detect oxidative damage or monitor protein structural alterations in diseases like neurodegenerative disorders, cardiovascular diseases, or cancer. Additionally, DTNB antibodies serve as tools to evaluate drug effects on cellular redox homeostasis or to validate thiol-blocking experimental conditions.

Their specificity for TNB-labeled epitopes makes them valuable in distinguishing oxidized versus reduced protein forms, aiding research on oxidative stress mechanisms and antioxidant defense systems. Proper controls are required to avoid cross-reactivity with other disulfide-bonded structures.