中文名稱:CYP11A1抗體 | 英文名稱:Rabbit Polyclonal CYP11A1 Antibody |
品牌: 艾普蒂 | 產(chǎn)地: 武漢 |
保存條件: Stored at -20°C for 1905 year. Avoid repeated freeze / thaw cycles. | 產(chǎn)品類別: 抗體 |
重組: 否 | 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA |
種屬反應(yīng)性: Human,Mouse,Rat | 宿主: Rabbit |
偶聯(lián)物: 無 | 靶點(diǎn): CYP11A1 |
WB | 咨詢技術(shù) | Human,Mouse,Rat |
IF | 咨詢技術(shù) | Human,Mouse,Rat |
IHC | 咨詢技術(shù) | Human,Mouse,Rat |
ICC | 技術(shù)咨詢 | Human,Mouse,Rat |
FCM | 咨詢技術(shù) | Human,Mouse,Rat |
Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
Aliases | CYP11A; CYPXIA1; P450SCC |
WB Predicted band size | 60 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Fusion protein of human CYP11A1 |
Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Human normal kidney tissue, Primary antibody: P01254(CYP11A1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes
以下是關(guān)于CYP11A1抗體的3篇參考文獻(xiàn)示例(注:內(nèi)容為虛構(gòu),僅作演示用途):
1. **文獻(xiàn)名稱**: "Immunolocalization of CYP11A1 in Human Adrenal Cortex and Its Role in Steroidogenesis"
**作者**: Miller WL, Auchus RJ
**摘要**: 本研究利用CYP11A1抗體通過免疫組化技術(shù)定位其在人腎上腺皮質(zhì)細(xì)胞中的表達(dá),證實(shí)其在線粒體中的分布,并探討其在皮質(zhì)醇和醛固酮合成中的關(guān)鍵作用。
2. **文獻(xiàn)名稱**: "Altered CYP11A1 Expression in Polycystic Ovary Syndrome Granulosa Cells"
**作者**: Sahmay S, Zhang X
**摘要**: 通過Western blot和免疫熒光分析,發(fā)現(xiàn)多囊卵巢綜合征患者的卵巢顆粒細(xì)胞中CYP11A1蛋白表達(dá)顯著降低,提示其與雌激素合成異常的相關(guān)性。
3. **文獻(xiàn)名稱**: "CYP11A1 Antibody Validation for Mitochondrial Protein Quantification in Aging Models"
**作者**: Pétervári E, Yoshioka H
**摘要**: 驗(yàn)證CYP11A1抗體的特異性及靈敏度,應(yīng)用于衰老小鼠模型,發(fā)現(xiàn)腎上腺和睪丸中線粒體CYP11A1蛋白水平隨年齡增長(zhǎng)而下降,影響類固醇激素穩(wěn)態(tài)。
4. **文獻(xiàn)名稱**: "CYP11A1 Mutations and Antibody-Based Detection in Congenital Adrenal Hyperplasia"
**作者**: Bose HS, Huang MC
**摘要**: 開發(fā)高特異性CYP11A1抗體用于先天性腎上腺皮質(zhì)增生癥患者的突變蛋白檢測(cè),揭示部分病例中酶功能缺陷與膽固醇代謝障礙的關(guān)聯(lián)。
The CYP11A1 antibody is a crucial tool for studying the enzyme cytochrome P450 family 11 subfamily A member 1 (CYP11A1), also known as cholesterol side-chain cleavage enzyme (P450scc). This mitochondrial enzyme catalyzes the first and rate-limiting step in steroid hormone biosynthesis by converting cholesterol to pregnenolone. It is expressed primarily in steroidogenic tissues, including the adrenal glands, gonads, and placenta, making it essential for producing glucocorticoids, mineralocorticoids, and sex hormones.
CYP11A1 antibodies are widely used in research to detect and quantify the enzyme’s expression and localization via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help investigate disorders linked to steroidogenesis, such as congenital adrenal hyperplasia, adrenal insufficiency, and reproductive endocrine disorders. They also aid in exploring CYP11A1’s role in pathological conditions, including cancers where steroidogenic pathways are dysregulated.
Most CYP11A1 antibodies target specific epitopes, such as the N-terminal region or conserved catalytic domains, and are often raised in rabbits or mice. Validation includes testing on tissues or cell lines with known CYP11A1 expression. Researchers rely on these antibodies to study regulatory mechanisms of steroidogenesis, enzyme inhibition, and tissue-specific expression patterns, contributing to advancements in endocrinology, reproductive biology, and therapeutic development.
成立日期 | 2024-06-24 (2年) | 注冊(cè)資本 | 50萬(元) |
員工人數(shù) | 1-10人 | 年?duì)I業(yè)額 | ¥ 100萬以內(nèi) |
主營(yíng)行業(yè) | 中間體,化學(xué)試劑 | 經(jīng)營(yíng)模式 | 貿(mào)易,工廠,試劑,定制,服務(wù) |
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