中文名稱:LAMP2抗體 | 英文名稱:Rabbit Polyclonal LAMP2 Antibody |
品牌: 艾普蒂 | 產(chǎn)地: 武漢 |
保存條件: Stored at -20°C for 1662 year. Avoid repeated freeze / thaw cycles. | 產(chǎn)品類別: 抗體 |
重組: 否 | 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA |
種屬反應(yīng)性: Human,Mouse,Rat | 宿主: Rabbit |
偶聯(lián)物: 無 | 靶點(diǎn): LAMP2 |
WB | 咨詢技術(shù) | Human,Mouse,Rat |
IF | 咨詢技術(shù) | Human,Mouse,Rat |
IHC | 1/50-1/200 | Human,Mouse,Rat |
ICC | 技術(shù)咨詢 | Human,Mouse,Rat |
FCM | 咨詢技術(shù) | Human,Mouse,Rat |
Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
Aliases | LAMPB; CD107b; LAMP-2; LGP110 |
WB Predicted band size | 45 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Fusion protein of human LAMP2 |
Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane: Human placenta tissue, Primary antibody: P00320(LAMP2 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
The image is immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using P00320(LAMP2 Antibody) at dilution 1/40. (Original magnification: ×200)
The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P00320(LAMP2 Antibody) at dilution 1/40. (Original magnification: ×200)
以下是關(guān)于LAMP2抗體的3-4篇參考文獻(xiàn)及其簡要摘要:
1. **"LAMP-2 deficiency leads to impaired autophagy and Danon disease"**
- **作者**: Tanaka Y, et al.
- **摘要**: 該研究揭示了LAMP2蛋白在自噬過程中的關(guān)鍵作用,LAMP2基因突變導(dǎo)致Danon病(一種罕見的溶酶體貯積癥),表現(xiàn)為心肌病和骨骼肌異常。研究通過小鼠模型證明LAMP2缺失會(huì)導(dǎo)致自噬體與溶酶體融合障礙。
2. **"LAMP2 regulates autophagy in the mammalian heart"**
- **作者**: Nishino I, et al.
- **摘要**: 本文通過LAMP2抗體在心臟組織中的免疫染色分析,發(fā)現(xiàn)LAMP2缺失會(huì)導(dǎo)致心臟細(xì)胞自噬功能異常,進(jìn)一步闡明了其在心臟病理中的重要性,并與人類Danon病的臨床表現(xiàn)相關(guān)聯(lián)。
3. **"Antibody-based profiling of lysosomal membrane proteins in human tissues"**
- **作者**: Eskelinen EL, et al.
- **摘要**: 研究開發(fā)了針對溶酶體膜蛋白(包括LAMP2)的特異性抗體,用于免疫印跡和免疫組化分析,驗(yàn)證了LAMP2在多種組織中的表達(dá)模式及其在溶酶體功能研究中的應(yīng)用價(jià)值。
4. **"Role of LAMP2 in lysosomal biogenesis and pathogen clearance"**
- **作者**: Saftig P, et al.
- **摘要**: 該文獻(xiàn)利用LAMP2抗體研究其在溶酶體成熟和病原體降解中的作用,發(fā)現(xiàn)LAMP2缺失會(huì)損害溶酶體酸化過程,影響細(xì)胞內(nèi)病原體(如細(xì)菌)的清除能力。
這些文獻(xiàn)涵蓋了LAMP2的分子功能、疾病機(jī)制及實(shí)驗(yàn)工具應(yīng)用,可作為相關(guān)研究的核心參考。
LAMP2 (Lysosomal-Associated Membrane Protein 2) is a key transmembrane glycoprotein predominantly located in lysosomal and late endosomal membranes. It belongs to the LAMP family, which includes LAMP1 and LAMP2. both critical for maintaining lysosomal structural integrity and function. LAMP2 consists of two domains: a heavily glycosylated luminal domain, which shields the lysosomal membrane from degradation, and a short cytoplasmic tail involved in intracellular trafficking and organelle interactions.
This protein plays multifaceted roles in autophagy, lysosomal enzyme activity regulation, and antigen presentation. Notably, LAMP2 is essential for autophagosome-lysosome fusion during autophagy, a cellular waste clearance process. Three splice variants (LAMP2A, LAMP2B, LAMP2C) exist, with LAMP2A specifically linked to chaperone-mediated autophagy.
Mutations in the LAMP2 gene are associated with Danon disease, a rare X-linked disorder characterized by cardiomyopathy, skeletal myopathy, and intellectual disability. LAMP2 antibodies are widely used in research to study lysosomal dynamics, autophagy-related mechanisms, and disease pathology. They also aid in diagnosing lysosomal storage disorders and monitoring protein expression in cancers or neurodegenerative diseases. Commercial LAMP2 antibodies typically target conserved regions of the luminal domain, enabling applications in Western blot, immunofluorescence, and immunohistochemistry.
成立日期 | 2024-06-24 (2年) | 注冊資本 | 50萬(元) |
員工人數(shù) | 1-10人 | 年?duì)I業(yè)額 | ¥ 100萬以內(nèi) |
主營行業(yè) | 中間體,化學(xué)試劑 | 經(jīng)營模式 | 貿(mào)易,工廠,試劑,定制,服務(wù) |
產(chǎn)品名稱 | 價(jià)格 | 公司名稱 | 報(bào)價(jià)日期 | |
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¥1280 |
湖北艾普蒂生物工程有限公司
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2025-06-04 | ||
¥1098 |
VIP3年
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上海滬震實(shí)業(yè)有限公司
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2025-08-01 | |
¥960.00 |
VIP10年
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北京索萊寶科技有限公司
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2025-07-30 |