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DDX1抗體,Mouse Monoclonal DDX1 Antibody
  • DDX1抗體,Mouse Monoclonal DDX1 Antibody
  • DDX1抗體,Mouse Monoclonal DDX1 Antibody
  • DDX1抗體,Mouse Monoclonal DDX1 Antibody

DDX1抗體

價格 詢價
包裝 20μl 50μl 100μl
最小起訂量 1μl
發(fā)貨地 湖北
更新日期 2025-05-10
QQ交談 微信洽談

產(chǎn)品詳情

中文名稱:DDX1抗體英文名稱:Mouse Monoclonal DDX1 Antibody
品牌: 艾普蒂產(chǎn)地: 武漢
保存條件: Stored at -20°C for 883 year. Avoid repeated freeze / thaw cycles.產(chǎn)品類別: 抗體
重組: 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA
種屬反應(yīng)性: Human,Mouse,Rat 宿主: Mouse
偶聯(lián)物: 靶點: DDX1
2025-05-10 DDX1抗體 Mouse Monoclonal DDX1 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武漢 Stored at -20°C for 883 year. Avoid repeated freeze / thaw cycles. 抗體

驗證與應(yīng)用

應(yīng)用及物種
WB咨詢技術(shù) Human,Mouse,Rat
IF咨詢技術(shù) Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/200 - 1/1000 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

產(chǎn)品詳情

AliasesDBP-RB; UKVH5d
Entrez GeneID1653
clone3E5B2
WB Predicted band size82.4kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human DDX1 (AA: 642-740) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


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       Western blot analysis using DDX1 mouse mAb against Hela (1), MCF-7 (2), A431 (3), PC-3 (4), and Jurkat (5) cell lysate.    


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       Immunofluorescence analysis of Hela cells using DDX1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.    


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       Immunofluorescence analysis of Hela cells using DDX1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Flow cytometric analysis of Hela cells using DDX1 mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using DDX1 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using DDX1 mouse mAb with DAB staining.    


           

參考文獻

以下是關(guān)于DDX1抗體的3篇參考文獻示例,涵蓋其在癌癥、病毒機制及信號通路中的研究應(yīng)用:

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1. **文獻名稱**:*Amplification of DDX1. a putative DEAD box RNA helicase gene, in neuroblastoma*

**作者**:Godbout, R., et al.

**摘要**:本研究揭示了DDX1基因在神經(jīng)母細(xì)胞瘤中與MYCN基因共擴增的現(xiàn)象。通過DDX1特異性抗體進行免疫組化分析,發(fā)現(xiàn)其在腫瘤組織中的高表達與患者預(yù)后相關(guān),提示DDX1可能作為癌癥治療的潛在靶點。

2. **文獻名稱**:*DDX1 interacts with HIV-1 Tat to promote viral transcription*

**作者**:Edgcomb, S.P., et al.

**摘要**:該研究探討了DDX1在HIV-1病毒復(fù)制中的關(guān)鍵作用。利用DDX1抗體進行免疫共沉淀(Co-IP)和免疫熒光實驗,證實DDX1與病毒蛋白Tat直接互作,促進病毒RNA的轉(zhuǎn)錄與輸出,為抗病毒治療提供了新思路。

3. **文獻名稱**:*DDX1 drives Wnt/β-catenin signaling in colorectal cancer by regulating mRNA stability*

**作者**:Huang, Y., et al.

**摘要**:文章通過Western blot和免疫組化技術(shù)(使用DDX1抗體)發(fā)現(xiàn),結(jié)直腸癌中DDX1表達上調(diào)可通過穩(wěn)定Wnt通路相關(guān)mRNA促進腫瘤進展。敲低DDX1顯著抑制癌細(xì)胞增殖,表明其作為治療靶點的潛力。

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**備注**:以上文獻信息基于領(lǐng)域內(nèi)常見研究方向整理,具體引用時建議通過PubMed或Google Scholar核對作者、標(biāo)題及摘要準(zhǔn)確性。

       

背景信息

The DDX1 antibody is a key tool for studying the DEAD-box helicase 1 (DDX1) protein, a member of the DEAD-box RNA helicase family characterized by the conserved Asp-Glu-Ala-Asp (DEAD) motif. DDX1 plays critical roles in RNA metabolism, including transcription, splicing, RNA transport, and viral RNA processing. It is also implicated in maintaining genomic stability and DNA repair. Dysregulation of DDX1 has been linked to cancers, particularly neuroblastoma, where its overexpression correlates with poor prognosis and chemoresistance. Additionally, DDX1 interacts with HIV-1 RNA and facilitates viral replication, making it a potential therapeutic target.

DDX1 antibodies are widely used in research to detect and quantify DDX1 expression in cells or tissues via techniques like Western blotting, immunohistochemistry, immunofluorescence, and co-immunoprecipitation. These antibodies help elucidate DDX1’s subcellular localization, interactions with nucleic acids or proteins, and functional mechanisms in both physiological and pathological contexts. Commercial DDX1 antibodies are typically raised in rabbits or mice, targeting specific epitopes (e.g., N-terminal or C-terminal regions) to ensure specificity. Validation data, including knockout/knockdown controls, are essential to confirm antibody reliability. Ongoing research leverages DDX1 antibodies to explore its roles in cancer biology, antiviral immunity, and RNA-related diseases, highlighting their importance in translational and basic science.

       
關(guān)鍵字: DDX1抗體;DDX1;DDX1 Antibody;

公司簡介

湖北瑞和寧生物科技有限公司研究創(chuàng)新能力, 擁有完整、科學(xué)、質(zhì)量管理體系。并不斷致力于技術(shù)創(chuàng)新,產(chǎn)品創(chuàng)新和管理創(chuàng)新,贏得了國內(nèi)外廣大用戶的信任。
成立日期 2024-06-24 (2年) 注冊資本 50萬(元)
員工人數(shù) 1-10人 年營業(yè)額 ¥ 100萬以內(nèi)
主營行業(yè) 中間體,化學(xué)試劑 經(jīng)營模式 貿(mào)易,工廠,試劑,定制,服務(wù)
  • 湖北瑞和寧生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注冊資本:50萬(元)
  • 企業(yè)類型:有限責(zé)任公司(自然人投資或控股)
  • 主營產(chǎn)品:中間體。試劑
  • 公司地址:江漢區(qū)
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