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RPTOR抗體,Mouse Monoclonal RPTOR Antibody
  • RPTOR抗體,Mouse Monoclonal RPTOR Antibody
  • RPTOR抗體,Mouse Monoclonal RPTOR Antibody
  • RPTOR抗體,Mouse Monoclonal RPTOR Antibody

RPTOR抗體

價(jià)格 詢價(jià)
包裝 20μl 50μl 100μl
最小起訂量 1μl
發(fā)貨地 湖北
更新日期 2025-05-10
QQ交談 微信洽談

產(chǎn)品詳情

中文名稱:RPTOR抗體英文名稱:Mouse Monoclonal RPTOR Antibody
品牌: 艾普蒂產(chǎn)地: 武漢
保存條件: Stored at -20°C for 666 year. Avoid repeated freeze / thaw cycles.產(chǎn)品類別: 抗體
重組: 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA
種屬反應(yīng)性: Human,Mouse,Rat 宿主: Mouse
偶聯(lián)物: 靶點(diǎn): RPTOR
2025-05-10 RPTOR抗體 Mouse Monoclonal RPTOR Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武漢 Stored at -20°C for 666 year. Avoid repeated freeze / thaw cycles. 抗體

驗(yàn)證與應(yīng)用

應(yīng)用及物種
WB咨詢技術(shù) Human,Mouse,Rat
IF咨詢技術(shù) Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/200 - 1/1000 Human,Mouse,Rat
FCM咨詢技術(shù) Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

產(chǎn)品詳情

AliasesKOG1; Mip1
Entrez GeneID57521
clone5A3A4
WB Predicted band size149kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human RPTOR (AA: 874-1009) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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       Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    


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       Immunofluorescence analysis of HepG2 cells using RPTOR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)    


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       Immunohistochemical analysis of paraffin-embedded human muscle tissues using RPTOR mouse mAb with DAB staining.    


           

參考文獻(xiàn)

以下是3-4篇關(guān)于RPTOR抗體的參考文獻(xiàn),按文獻(xiàn)名稱、作者和摘要內(nèi)容概括整理:

1. **文獻(xiàn)名稱**:*Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton*

**作者**:Sarbassov, D.D. et al. (2004)

**摘要**:研究首次發(fā)現(xiàn)RPTOR(raptor)與mTOR形成復(fù)合物(mTORC1),并利用特異性抗體驗(yàn)證其在調(diào)控細(xì)胞生長中的作用??贵w用于免疫共沉淀實(shí)驗(yàn),揭示RPTOR在營養(yǎng)信號(hào)傳導(dǎo)中的關(guān)鍵功能。

2. **文獻(xiàn)名稱**:*PRAS40 regulates mTORC1 kinase activity by functioning as a direct inhibitor of substrate binding*

**作者**:Sancak, Y. et al. (2007)

**摘要**:通過RPTOR抗體進(jìn)行免疫印跡(Western blot)和免疫熒光分析,證明PRAS40通過結(jié)合RPTOR抑制mTORC1活性,揭示其在能量應(yīng)激條件下的調(diào)控機(jī)制。

3. **文獻(xiàn)名稱**:*Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action in cells*

**作者**:Hara, K. et al. (2002)

**摘要**:早期研究利用RPTOR抗體驗(yàn)證其在mTOR復(fù)合物中的結(jié)構(gòu)作用,發(fā)現(xiàn)RPTOR缺失導(dǎo)致mTOR底物(如S6K1)磷酸化水平下降,證實(shí)其對(duì)mTORC1功能不可或缺。

4. **文獻(xiàn)名稱**:*mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery*

**作者**:Kim, D.H. et al. (2002)

**摘要**:通過RPTOR特異性抗體進(jìn)行免疫沉淀實(shí)驗(yàn),證明RPTOR-mTOR復(fù)合物對(duì)氨基酸和生長因子敏感,調(diào)控蛋白質(zhì)合成和細(xì)胞增殖。

這些文獻(xiàn)均通過RPTOR抗體在分子機(jī)制研究中驗(yàn)證其功能,涵蓋免疫沉淀、Western blot和復(fù)合物分析等實(shí)驗(yàn)方法。

       

背景信息

The RPTOR (Regulatory-Associated Protein of mTOR) antibody is a crucial tool in studying the mechanistic target of rapamycin (mTOR) signaling pathway, which regulates cell growth, proliferation, and metabolism. RPTOR, a core component of mTOR complex 1 (mTORC1), acts as a scaffold protein that recruits substrates and facilitates mTORC1 activation in response to nutrients, growth factors, and cellular energy status. Dysregulation of RPTOR-mediated mTORC1 signaling is implicated in cancer, metabolic disorders, and aging.

The RPTOR antibody enables researchers to detect and quantify RPTOR protein expression levels, assess its post-translational modifications (e.g., phosphorylation), and investigate its subcellular localization. It is widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence. Specific RPTOR antibodies are validated for species cross-reactivity (e.g., human, mouse, rat) and tested for specificity using knockout controls to minimize off-target signals.

In research, this antibody aids in exploring mTORC1's role in diseases, drug discovery (e.g., mTOR inhibitors), and mechanistic studies of nutrient-sensing pathways. For example, it helps evaluate RPTOR expression changes under conditions like starvation, growth factor stimulation, or pharmacological inhibition. Its application extends to cancer biology, where mTORC1 hyperactivation is common, and to metabolic studies linking RPTOR to insulin signaling and autophagy regulation. Validation data, including molecular weight confirmation (~150 kDa) and functional assays, are critical for ensuring experimental reliability.

       
關(guān)鍵字: RPTOR抗體;RPTOR;RPTOR Antibody;

公司簡介

湖北瑞和寧生物科技有限公司研究創(chuàng)新能力, 擁有完整、科學(xué)、質(zhì)量管理體系。并不斷致力于技術(shù)創(chuàng)新,產(chǎn)品創(chuàng)新和管理創(chuàng)新,贏得了國內(nèi)外廣大用戶的信任。
成立日期 2024-06-24 (2年) 注冊(cè)資本 50萬(元)
員工人數(shù) 1-10人 年?duì)I業(yè)額 ¥ 100萬以內(nèi)
主營行業(yè) 中間體,化學(xué)試劑 經(jīng)營模式 貿(mào)易,工廠,試劑,定制,服務(wù)
  • 湖北瑞和寧生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注冊(cè)資本:50萬(元)
  • 企業(yè)類型:有限責(zé)任公司(自然人投資或控股)
  • 主營產(chǎn)品:中間體。試劑
  • 公司地址:江漢區(qū)
詢盤

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RPTOR抗體相關(guān)廠家報(bào)價(jià)

產(chǎn)品名稱 價(jià)格   公司名稱 報(bào)價(jià)日期
詢價(jià)
VIP3年
維百奧(北京)生物科技有限公司
2025-08-01
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VIP1年
費(fèi)雪(杭州)醫(yī)學(xué)研究有限公司
2025-07-28
詢價(jià)
上海華盈生物醫(yī)藥科技有限公司
2025-08-02
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