+ +

•  

   

     Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);    

  
  

•  

   

     Immunofluorescence analysis of Hela cells using LHX2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    

  
  

The LHX2 antibody is a crucial tool in developmental biology and neuroscience research, targeting the LIM Homeobox 2 (LHX2) protein, a member of the LIM-homeodomain transcription factor family. LHX2 plays pivotal roles in embryonic development, particularly in nervous system patterning, retinal development, and organogenesis. It regulates gene expression by binding DNA through its homeodomain and interacting with other proteins via its LIM domains. Studies highlight its involvement in maintaining neural stem cell populations, guiding axonal projections, and ensuring proper differentiation of sensory organs.

Researchers use LHX2 antibodies primarily in techniques like immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting to visualize protein expression and localization in tissues or cultured cells. These applications have revealed LHX2's dynamic expression in the developing brain, eye, and hematopoietic systems. Aberrant LHX2 expression is linked to pathologies, including glioblastoma and breast cancer, making its antibody a valuable probe for oncological studies. Commercial LHX2 antibodies are typically raised in rabbits or mice, validated for specificity using knockout controls or siRNA-mediated knockdown. Their utility spans basic developmental studies, disease mechanism exploration, and potential therapeutic target identification.