JC-1 NEW
| Price | $59 | $81 | $153 |
| Package | 1mg | 2mg | 5mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-07-24 |
Product Details
| Product Name: JC-1 | CAS No.: 3520-43-2 |
| Purity: 99.58% | Supply Ability: 10g |
| Release date: 2025/07/24 |
Product Introduction
Bioactivity
| Name | JC-1 |
| Description | JC-1 (CBIC2) is a fluorescent lipophilic carbocyanine dye. It has been used to measure mitochondrial membrane potential. JC 1 can be used in a probe for measuring mitochondrial membrane potential by flow cytometry. |
| Cell Research | Instructions I. Solution preparation 1. Stock solution preparation: JC-1 is dissolved in DMSO to prepare a stock solution. The concentration of the stock solution is usually 5 mg/ml. Please refer to the manufacturer's instructions for the specific concentration. 2. Working concentration: The working concentration of JC-1 is usually 1-20 μg/ml, but the optimal concentration may need to be optimized depending on the cell type and experimental conditions. 3. Dilution: Dilute the stock solution with cell culture medium or appropriate buffer before use. II. Staining cells 1. Cell preparation: Culture the cells to be tested under appropriate conditions (e.g., 37°C, 5% CO?). 2. Incubation with JC-1: Add the diluted JC-1 working solution to the cells. Depending on the cell type and experimental conditions, incubate for 15-30 minutes. 3. Washing: After incubation, wash the cells 1-2 times with preheated cell culture medium or PBS to remove excess dye. 3. Fluorescence detection 1. Fluorescence microscopy: Use a fluorescence microscope equipped with appropriate filters to detect green (monomer JC-1) and red (aggregate JC-1) fluorescence. The excitation wavelength of green fluorescence is usually 485-490 nm, and that of red fluorescence is 525-530 nm, with emission peaks at 535 nm (green) and 590 nm (red), respectively. 2. Flow cytometry: Flow cytometry can also be used for quantitative analysis. Use appropriate filters (e.g., 525/40 nm for green fluorescence and 585/42 nm for red fluorescence) for detection. 3. Data interpretation: An increase in red fluorescence indicates a higher mitochondrial membrane potential, while an increase in green fluorescence indicates a loss or decrease in membrane potential. |
| In vitro | JC-1 fluorescence is usually excited by the 488nm laser wavelength common in flow cytometers[1]. JC-1 (2.5μM) exposed to murine L1210 lymphoblasts, can be detected the presence of both cytoplasmic JC-1 monomer and mitochondrial J-aggregates in these cells. Fluorescent labeling of mitochondria with either JC-1 (1 μg/mL, 15 min), reveals that are distributed irregularly, resulting in regions of high and low mitochondrial content within astrocytes[2]. JC-1 is avidly accumulated in sensitive K562 cells where it displays both a green cytoplasmic and red mitochondrial fluorescence. JC-1 is poorly accumulated in resistant K562 cells, which displays only a slight green fluorescence[4]. JC-1 has been shown to interact with α-synuclein at the acidic C-terminal region with a Kd of 2.6 μM. JC-1 itself does not accelerate the protein aggregation of α-synuclein in the absence of iron, insted, it decelerates the aggregation process by extending the lag phase approx[3]. |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 14 mg/mL (21.46 mM), Sonication is recommended. |
| Keywords | JC-1 | JC1 | JC 1 | Inhibitor | inhibit | CBIC-2 | CBIC 2 |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
- Address: 36 Washington Street, Wellesley Hill, MA
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