7-Ethoxyresorufin NEW
| Price | $30 | $38 | $61 |
| Package | 1mg | 2mg | 5mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-07-28 |
Product Details
| Product Name: 7-Ethoxyresorufin | CAS No.: 5725-91-7 |
| Purity: 99.35% | Supply Ability: 10g |
| Release date: 2025/07/28 |
Product Introduction
Bioactivity
| Name | 7-Ethoxyresorufin |
| Description | 7-Ethoxyresorufin (Resorufin ethyl ether) (7-ER) is a fluorometric substrate and competitive inhibitor of cytochrome P450, particularly CYP1A1. |
| Cell Research | Instructions I. Solution preparation 1. Stock solution preparation: 7-Ethoxyresorufin is dissolved in DMSO or ethanol and prepared to 1–10 mM (adjusted according to experimental needs). 2. Working concentration: Usually 0.5–5 μM. (Adjusted according to experimental needs) Notes: 1) Powder: Store dry and away from light at -20°C. 2) Stock solution: Store away from light at -20°C, and can be stored at 4°C for a short period of time to avoid repeated freezing and thawing. II. CYP1A1/CYP1A2 enzyme activity detection Operation steps 1. Sample preparation: Use liver microsomes or cultured cells as samples. Cells need to be pretreated to induce the expression of CYP1A1 or CYP1A2 (such as treatment with benzo[a]pyrene or 3-methylcholanthracene). 2. Reaction system: 1) Prepare reaction buffer (e.g. 50 mM Tris-HCl, pH 7.4). 2) Dilute the stock solution of 7-Ethoxyresorufin to a working concentration (e.g. 2 μM) and add to the buffer. 3) Add NADPH generating system (e.g. NADPH or NADPH regenerating system) to start the reaction. 3. Reaction conditions: 1) Incubate at 37°C for 10–30 minutes (the specific time is optimized according to the experiment). 2) Stop the reaction: The reaction can be terminated by adding an equal volume of cold ethanol or methanol. 3. Fluorescence detection: Resorufin (deethoxylated product) generated by the reaction emits strong green fluorescence: Excitation wavelength: ~530 nm Emission wavelength: ~585 nm Measure the fluorescence signal using a fluorescence spectrophotometer or microplate reader. |
| In vivo | 7-ER inhibited responses to NO and nitrergic nerve stimulation through generation of superoxide radicals. A 7-ER sensitive P450 system may be involved in the bioactivation of GTN and SNP in rat aortic rings, but not in rabbit aorta or rat anococcygeus muscles. |
| Storage | store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : < 1 mg/mL (insoluble or slightly soluble) DMF : 1.92 mg/mL (7.96 mM), Sonication is recommended. |
| Keywords | substrate | NOSynthase | NOS | NO Synthase | Nitric oxide synthases | Inhibitor | inhibit | Fluorometric | Cytochrome P450 | CYPs | CYP1A1 | 7-Ethoxyresorufin | 7Ethoxyresorufin | 7 Ethoxyresorufin |
| Inhibitors Related | Tebuconazole | Naringin dihydrochalcone | L-NAME hydrochloride | Chondroitin sulfate | Mifepristone | Apigenin | Fenofibrate | Ethyl cinnamate | Naringin | Naringenin | Tauroursodeoxycholate | Chlorzoxazone |
| Related Compound Libraries | Neuroprotective Compound Library | Anti-Aging Compound Library |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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