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4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure

HEPES Struktur
7365-45-9
CAS-Nr.
7365-45-9
Bezeichnung:
4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure
Englisch Name:
HEPES
Synonyma:
HEPES BUFFER;4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID;hepes solution;HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID;Biological Buffer;HEPES buffer Solution;4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID;4-(2-hydroxyethyl)piperazin-1-ylethanesulphonic acid;2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID;EPES
CBNumber:
CB5408557
Summenformel:
C8H18N2O4S
Molgewicht:
238.3
MOL-Datei:
7365-45-9.mol

4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure Eigenschaften

Schmelzpunkt:
234-238 °C
Siedepunkt:
408℃[at 101 325 Pa]
chüttdichte
560kg/m3
Dichte
1.07 g/mL at 20 °C
Dampfdruck
0Pa at 25℃
Brechungsindex
n20/D 1.339
storage temp. 
2-8°C
L?slichkeit
H2O: 1 M at 20 °C, clear, colorless
Aggregatzustand
powder
Farbe
White
PH
5.0-6.5 (1Maqueous solution)
pka
7.5(at 25℃)
S?ure-Base-Indikators(pH-Indikatoren)
7.0 - 7.6
Biologische Quelle
synthetic
Wasserl?slichkeit
Soluble
maximale Wellenl?nge (λmax)
λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04
Merck 
14,4654
BRN 
883043
Stabilit?t:
Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture.
InChIKey
JKMHFZQWWAIEOD-UHFFFAOYSA-N
LogP
-3.85 at 20℃
CAS Datenbank
7365-45-9(CAS DataBase Reference)
EPA chemische Informationen
1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- (7365-45-9)
Absorption
≤0.05 at 290 in H2O at 33%
Sicherheit
  • Risiko- und Sicherheitserkl?rung
  • Gefahreninformationscode (GHS)
Kennzeichnung gef?hrlicher Xi
R-S?tze: 36/37/38
S-S?tze: 24/25-22-36-26
WGK Germany  1
RTECS-Nr. TL6809000
TSCA  Yes
HS Code  29335995
Bildanzeige (GHS) GHS hazard pictograms
Alarmwort Warnung
Gefahrenhinweise
Code Gefahrenhinweise Gefahrenklasse Abteilung Alarmwort Symbol P-Code
H302 Gesundheitssch?dlich bei Verschlucken. Akute Toxizit?t oral Kategorie 4 Warnung GHS hazard pictogramssrc="/GHS07.jpg" width="20" height="20" /> P264, P270, P301+P312, P330, P501
H315 Verursacht Hautreizungen. Hautreizung Kategorie 2 Warnung GHS hazard pictogramssrc="/GHS07.jpg" width="20" height="20" /> P264, P280, P302+P352, P321,P332+P313, P362
H319 Verursacht schwere Augenreizung. Schwere Augenreizung Kategorie 2 Warnung GHS hazard pictogramssrc="/GHS07.jpg" width="20" height="20" /> P264, P280, P305+P351+P338,P337+P313P
H335 Kann die Atemwege reizen. Spezifische Zielorgan-Toxizit?t (einmalige Exposition) Kategorie 3 (Atemwegsreizung) Warnung GHS hazard pictogramssrc="/GHS07.jpg" width="20" height="20" />
Sicherheit
P261 Einatmen von Staub vermeiden.
P271 Nur im Freien oder in gut belüfteten R?umen verwenden.
P280 Schutzhandschuhe/Schutzkleidung/Augenschutz tragen.

4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure Chemische Eigenschaften,Einsatz,Produktion Methoden

R-S?tze Betriebsanweisung:

R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.

S-S?tze Betriebsanweisung:

S24/25:Berührung mit den Augen und der Haut vermeiden.
S22:Staub nicht einatmen.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.

Beschreibung

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Chemische Eigenschaften

White crystalline powder

Verwenden

Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.

Definition

ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2.

Allgemeine Beschreibung

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

Biologische Aktivit?t

HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.

l?uterung methode

Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.]

4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure Upstream-Materialien And Downstream Produkte

Upstream-Materialien

2-Piperazin-1-ylethanol Natrium-2-hydroxyethansulfonat

Downstream Produkte

4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure Anbieter Lieferant Produzent Hersteller Vertrieb H?ndler.

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7365-45-9(4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure)Verwandte Suche:

  • HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID MOLECULAR BIOLOGY GRADE
  • HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID 99+%
  • N-(2-HYDROXYETHYL)-PIPERAZINE-N'-2-ETHANESULFONIC ACID(4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID(HEPES-ACID))
  • Hepes,FreeAcid(N-[2-Hydroxyethyl]Piperaz
  • 1-HeptaneSulfonicAcidSod.SaltGr
  • N-(2-Hydroxyethyl)Piperazine-N'-(3-EthanesulfonicAcid)
  • N-(2-Hydroxyethyl)Piperazine-N'-(2-EthanesulfonicAcid)(Hepes)
  • Hepes,LowSodium,FreeAcid
  • HEPES free acid, DNase, Rnase and Protease free, for molecular biology, 99+%
  • Hydroxyethyl)-1-piperazineethyanesulfonic acid, 4-(2-
  • 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid(HEPES)
  • 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid
  • 4-(2-hydroxyerhyl)piperazine-1-erhaesulfonic acid
  • HEPES, for biochemistry, 99%
  • 4-(2-Hydroxyethyl)-1-Piperazin
  • Hydroxyethyl)piperazine-N'-2-ethane sulfonic acid
  • 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-
  • N-(2-hydroxymethyl)piperazine-N｀-(2-ethanesulfonic acid)(HEPES)
  • N-2-HYDROXYETHYLPIPERAZINE-N-2-ETHANSULFONICACID(HEPES)
  • 4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure
  • 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid HEPES
  • HEPES / 4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULPHONIC ACID
  • HEPES (4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID)
  • HEPES ULTRAPURE
  • N-2-HYDROXYETHYLPIPERAZINE-N''-2-ETHANE SULPHONIC ACID >99.5%
  • N-[2-HYDROXYERHYL]PIPERAZINE-N''-ETHANESULFONICACID(HEPES)
  • HEPES free acid ultrapure
  • N-2-Hydroxyethylpiperazine-N'-2-ethanesulphonic acid, low sodium >99.5%
  • HEPES(N-[2-Hydroxyethyl]piperazine-N'-[Ethanesulfonic acid]),Free Acid
  • N'-2-N-2-hydroxyethyl piperazineethanesulfonic acid
  • HEPES, >=99.5% (titration)
  • HEPES SynonyMs 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
  • HEPES,4-(2-hydroxyerhyl)piperazine-1-erhanesulfonic acid
  • HEPES Vetec(TM) reagent grade, 99.5%
  • 4-CHLORO-1-NAPHTHOL ULTRA PURE GRADE
  • POTASSIUM HYDROXIDE 0,05 MOL DUTY PAID
  • POTASSIUM HYDROXIDE 0,5MOL/L DUTY PAID
  • POTASSIUM HYDROXIDE 1 MOL/L DUTY PAID
  • SELENIUM STAND
  • MALATE DEHYDROGENASE ULTRA PURE GRADE
  • PHOSPHOTUNGSTIC ACID HIGH PURITY GRADE
  • 4-(2-hydroxyethyl)-1-piperazineethanesulfonicaci
  • hepesfreeacid
  • 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINE]ETHANESULFONIC ACID
  • LABOTEST-BB LT00138109
  • LABOTEST-BB LT00233156
  • HEPES
  • HEPES-ACID
  • 4-(2-Hydroxyethyl)-1-piperazineethanesulphonic acid
  • hepes free acid molecular biology*reagent
  • HEPES N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid)
  • HEPES, 1M solution
  • HEPES, Free Acid, MB Grade (1.12041)
  • HEPES, Free Acid, ULTROL Grade
  • HEPES, Molecular Biology Grade N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid), Molecular Biology Grade
  • HEPES, Tissue Culture Tested N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid), Tissue Culture Tested
  • N-(2-HYDROXYETHYL)PIPERAZINE N-2-ETHANESULFONIC ACID
  • N-2-HYDROXYETHYLPIPERAZINE-N'-2-ETHANE SULPHONIC ACID
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