| Identification | More | [Name]
HEPES | [CAS]
7365-45-9 | [Synonyms]
2-[4-(2-HYDROXYETHYL)-1-PIPERAZINE]ETHANESULFONIC ACID
2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID
4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID
4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID
GOOD'S BUFFER
HEPES
HEPES-ACID
HEPES BUFFER
HEPES BUFFERED SALINE
HEPES BUFFER PH 7.5
HEPES BUFFER PH 8.0
LABOTEST-BB LT00138109
LABOTEST-BB LT00233156
N-(2-HYDROXYETHYL)PIPERAZINE N-2-ETHANESULFONIC ACID
N-2-HYDROXYETHYLPIPERAZINE-N'-2-ETHANESULFONIC ACID
N-2-HYDROXYETHYLPIPERAZINE-N'-2-ETHANE SULPHONIC ACID
4-(2-hydroxyethyl)-1-piperazineethanesulfonicaci
hepesfreeacid
N-(2-Hydroxyethyl)piper
4-(2-Hydroxyethyl)-1-piperazineethanesulphonic acid | [EINECS(EC#)]
230-907-9 | [Molecular Formula]
C8H18N2O4S | [MDL Number]
MFCD00006158 | [Molecular Weight]
238.3 | [MOL File]
7365-45-9.mol |
| Chemical Properties | Back Directory | [Appearance]
White crystalline powder | [Melting point ]
234-238 °C
| [Boiling point ]
408℃[at 101 325 Pa] | [bulk density]
560kg/m3 | [density ]
1.07 g/mL at 20 °C
| [vapor pressure ]
0Pa at 25℃ | [refractive index ]
n20/D 1.339
| [storage temp. ]
2-8°C
| [solubility ]
H2O: 1 M at 20 °C, clear, colorless
| [form ]
powder
| [color ]
White | [PH]
5.0-6.5 (1Maqueous solution) | [PH Range]
7.0 - 7.6 | [pka]
7.5(at 25℃) | [Stability:]
Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture. | [biological source]
synthetic | [Water Solubility ]
Soluble | [λmax]
λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04 | [Detection Methods]
T,NMR | [Merck ]
14,4654 | [BRN ]
883043 | [InChIKey]
JKMHFZQWWAIEOD-UHFFFAOYSA-N | [LogP]
-3.85 at 20℃ | [CAS DataBase Reference]
7365-45-9(CAS DataBase Reference) | [EPA Substance Registry System]
7365-45-9(EPA Substance) | [Absorption]
≤0.05 at 290 in H2O at 33% |
| Safety Data | Back Directory | [Hazard Codes ]
Xi | [Risk Statements ]
R36/37/38:Irritating to eyes, respiratory system and skin . | [Safety Statements ]
S24/25:Avoid contact with skin and eyes .
S22:Do not breathe dust .
S36:Wear suitable protective clothing .
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . | [WGK Germany ]
1
| [RTECS ]
TL6809000
| [TSCA ]
Yes | [HS Code ]
29335995 |
| Hazard Information | Back Directory | [Description]
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. | [Chemical Properties]
White crystalline powder | [Uses]
Biological buffer. | [Uses]
Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability. | [Definition]
ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2. | [General Description]
HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions. | [Biological Activity]
Multi purpose buffer used in biological research. | [Synthesis]
The general procedure for the synthesis of 4-hydroxyethylpiperazineethanesulfonic acid from N-hydroxyethylpiperazine and sodium 2-hydroxyethylsulfonate is as follows: 0.5 mole of N-hydroxyethylpiperazine, 0.565 mole of sodium 2-hydroxyethylsulfonate, and 500 ml of methanol were added to a 1000 ml four-necked flask fitted with a refluxing condenser tube, and the condensation reaction was carried out under sufficient stirring. The reaction was first carried out at 50 °C for 2.5 minutes, then gradually heated to reflux and continued for 3.0 hours. The reaction was cooled after completion of the reaction to give a reaction mixture containing 4-hydroxyethylpiperazine ethanesulfonate. The yield of 4-hydroxyethylpiperazine ethanesulfonate was 93.6% as analyzed by high performance liquid chromatography. A 20 liter solution of 4-hydroxyethylpiperazine ethanesulfonate at a mass concentration of 15 wt% was diluted to 5 wt% with deionized water under stirring, followed by removal of sodium chloride by dialysis. Then the purified HEPES product was obtained by evaporation concentration, cooling crystallization and filtration. Finally, the product was washed once with anhydrous methanol and dried under vacuum to obtain purified HEPES.A sample of purified HEPES was dissolved and tested for purity, which showed a purity of 99.9%, a yield of 85.6%, a pH of 6.46, and a concentration of chloride ions of 6.8 μg/g. | [storage]
Room temperature | [Purification Methods]
Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.] | [References]
[1] Patent: CN106905262, 2017, A. Location in patent: Paragraph 0034; 0035; 0047 |
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