"RWPE-1 Thawing人正常前列腺上皮細(xì)胞系
細(xì)胞背景資料:腫瘤抑制基因: p53 + [PubMed: 9214605] pRB + [PubMed: 9214605] 一位正常男性前列腺組織切片的周圍區(qū)域的上皮細(xì)胞用單拷貝的人乳頭瘤病毒的18(HPV-18)進(jìn)行轉(zhuǎn)化,建立了RWPE-1 (ATCC CRL-11609) 細(xì)胞株 [PubMed: 9214605]. 在三維Matrigel培養(yǎng)時,在雄激素作用下,RWPE-1細(xì)胞形成腺胞并向培養(yǎng)基中分泌PSA。[PubMed: 11170142]. 當(dāng)與Matrigel或基質(zhì)細(xì)胞混合注射雄性裸鼠時,RWPE-1細(xì)胞也能形成腺胞[PubMed: 11304724] 并產(chǎn)生PSA。 來源于RWPE-1的細(xì)胞再用Kirstin鼠類腫瘤病毒轉(zhuǎn)染Ki-ras基因,建立了能成瘤的RWPE-2細(xì)胞株(ATCC CRL-11610) [PubMed: 9214605] 和 RWPE2-W99 (ATCC CRL-2853) 細(xì)胞株。 另外,用N--N-(MNU)處理RWPE-1,建立了一系列模擬前列腺癌進(jìn)程中不同時期的成瘤細(xì)胞株。 它們是 WPE1-NA22 (ATCC CRL-2849), WPE1-NB14 (ATCC CRL-2850, WPE1-NB11 (ATCC CRL-2851) 和 WPE1-NB26 (ATCC CRL-2852) 細(xì)胞株。 據(jù)提供者報道,RWPE-1 細(xì)胞株(ATCC CRL-11609)經(jīng)過檢測,乙肝、丙肝、人免疫缺陷病毒都呈陰性。
細(xì)胞形態(tài):上皮細(xì)胞樣
RWPE-1 Thawing人正常前列腺上皮細(xì)胞系
細(xì)胞生長:貼壁
接收細(xì)胞相關(guān)問題:如不能在收到細(xì)胞后及時操作細(xì)胞,T25及離心管發(fā)貨的細(xì)胞將可以消毒后在37度過夜,血清發(fā)貨的細(xì)胞在室溫下靜置1-2天(注意不要放冰箱),干冰發(fā)貨的可以在確認(rèn)干冰未完全升華時將細(xì)胞直接放回液氮或者-80度冰箱,若干冰完全升華,請即刻復(fù)蘇細(xì)胞。T25瓶及離心管在培養(yǎng)箱平衡是為了讓細(xì)胞盡快適應(yīng)培養(yǎng)箱環(huán)境,同時使部分因運(yùn)輸導(dǎo)致脫落的細(xì)胞貼壁,此步驟非常必要。T25瓶如果在顯微鏡下可以看到部分不貼壁的細(xì)胞,可以收集上清后離心(1200rpm,5min)后,將沉淀用新的培養(yǎng)基重懸后接種至新的培養(yǎng)瓶或皿中。部分細(xì)胞在運(yùn)輸過程中,由于不斷震蕩及環(huán)境不適,可能導(dǎo)致細(xì)胞破碎死亡,從而使培養(yǎng)基中漂浮很多細(xì)胞碎片及顆粒狀物質(zhì)。這種情況下,平衡后,貼壁細(xì)胞用PBS洗兩遍再加新的培養(yǎng)基培養(yǎng)或者傳代至新瓶中培養(yǎng)即可;懸浮細(xì)胞可以離心(1000rpm,3min)收集細(xì)胞,并用PBS重懸后再離心一次,再用新的培養(yǎng)基重懸并接種細(xì)胞。
細(xì)胞物種來源:人源或鼠源等其它物種來源
細(xì)胞產(chǎn)品包裝:復(fù)蘇形式:T25培養(yǎng)瓶(一瓶)或凍存形式:1ml凍存管(兩支)
細(xì)胞傳代方法:1:3傳代,2-3天傳一代。
細(xì)胞培養(yǎng)更好經(jīng)驗詳解:1)收到細(xì)胞,首先要鏡檢:觀察細(xì)胞形態(tài)是否正常,對于貼壁細(xì)胞則要注意看貼壁情況是否很好,觀察細(xì)胞密度,有疑議及時咨提供細(xì)胞的技術(shù)人員。 由于運(yùn)輸?shù)臅r間或者溫度的變化,很多貼壁細(xì)胞在盛滿培養(yǎng)基的瓶子里生長的狀態(tài)和平時會有點不一樣,主要是貼壁牢固問題。比如293T,平時貼壁就不是很牢,在裝滿的培養(yǎng)基瓶子里面,會發(fā)生大片的脫落,細(xì)胞聚集在一起,但是細(xì)胞生長還是正常的,通過離心收集,加以胰酶的消化,重新打散,又可以正常的貼壁生長。2)當(dāng)通過上一步的觀察,細(xì)胞初步?jīng)]有問題時,進(jìn)行下一步操作。當(dāng)收到的細(xì)胞密達(dá)到80%左右時,則處理如下:棄除瓶中大部分培養(yǎng)基,僅保留5到10mL培養(yǎng)所需的常規(guī)培養(yǎng)基;或更換新鮮的培養(yǎng)基,置于培養(yǎng)箱中,隨后每天觀察。 細(xì)胞在低于正常生長溫度情況下,會調(diào)整為靜止期,或者慢速生長期,恢復(fù)37度的環(huán)境至少3個小時左右,才能重新調(diào)整到接近對數(shù)生長期的狀態(tài),在對數(shù)生長期進(jìn)行細(xì)胞的傳代會大大增加傳代的成功率,和細(xì)胞的存活率。3)如果經(jīng)步觀察發(fā)現(xiàn)細(xì)胞密度超過90%,并且細(xì)胞狀態(tài)很好時,則復(fù)溫后2個小時需要立即傳代。傳代的比例應(yīng)依據(jù)不同的細(xì)胞而定。對于生長比較快,狀態(tài)穩(wěn)定,不易受外界影響的細(xì)胞可以一次多傳幾瓶;對于生長較慢,細(xì)胞比較薄,狀態(tài)容易波動的細(xì)胞類型,一次少傳些,每瓶細(xì)胞密度大些,便于穩(wěn)定生長。至于一些比較陌生的細(xì)胞,次處理也可以依照第二種情況。
細(xì)胞來源說明:細(xì)胞主要來源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少數(shù)國外著名大學(xué)建系
RWPE-1 Thawing人正常前列腺上皮細(xì)胞系
細(xì)胞生長特性:貼壁生長
【胰蛋白酶消化操作】1)加入消化液:小心吸出舊培養(yǎng)液,用PBS清洗(沖洗),加入適量消化液(胰蛋白酶液),注意消化液的量以蓋住細(xì)胞,消化溫度是37℃;2)顯微鏡下觀察細(xì)胞:倒置顯微鏡下觀察消化細(xì)胞,若胞質(zhì)回縮,細(xì)胞之間不再連接成片,表明此時細(xì)胞消化適度;(3)吸棄消化液加入培養(yǎng)液:棄去胰蛋白酶液,注意更換吸管,加入新鮮的培養(yǎng)液。【吹打分散細(xì)胞操作】1)吹打制懸:用滴管將已經(jīng)消化細(xì)胞吹打成細(xì)胞懸液;2)吸細(xì)胞懸液入離心管:將細(xì)胞懸液吸入10ml離心管中;3)平衡離心:平衡后將離心管放入臺式離心機(jī)中,以1000轉(zhuǎn)/分鐘離心6-8分鐘;4)棄上清液,加入新培養(yǎng)液:棄去上清液,加入2ml培養(yǎng)液,用滴管輕輕吹打細(xì)胞制成細(xì)胞懸液?!痉盅b稀釋細(xì)胞操作】1)顯微鏡下觀察細(xì)胞:倒置顯微鏡下觀察細(xì)胞量,必要是計數(shù)。注意密度過小會影響傳代細(xì)胞的生長,傳代細(xì)胞的密度應(yīng)該不低于5×105/ml。最后要做好標(biāo)記;2)分裝:將細(xì)胞懸液吸出分裝至2~3個培養(yǎng)瓶中,加入適量培養(yǎng)基旋緊瓶蓋;3)繼續(xù)培養(yǎng):用酒精棉球擦拭培養(yǎng)瓶,適當(dāng)旋松瓶蓋,放入CO2培養(yǎng)箱中繼續(xù)培養(yǎng)。傳代細(xì)胞2小時后開始貼附在瓶壁上。
細(xì)胞形態(tài)特性:上皮細(xì)胞
A-875細(xì)胞系相關(guān)產(chǎn)品:OVCA-433細(xì)胞、Helacyton gartleri細(xì)胞、MIA Paca2細(xì)胞
C6661細(xì)胞系相關(guān)產(chǎn)品:MOLP-8細(xì)胞、THLE-3細(xì)胞、NS1細(xì)胞
PC-3M-1E8細(xì)胞系相關(guān)產(chǎn)品:OVCAR-4細(xì)胞、ARH 77細(xì)胞、QBI-293A細(xì)胞
NW-38細(xì)胞系相關(guān)產(chǎn)品:Y3-Ag1.2.3細(xì)胞、BEAS-2B細(xì)胞、Mevo細(xì)胞
Laboratory of Allergic Diseases 2細(xì)胞系相關(guān)產(chǎn)品:SU-DHL-1細(xì)胞、Hep 3B2_1-7細(xì)胞、GM-215細(xì)胞
SKMel-28細(xì)胞系相關(guān)產(chǎn)品:Line 207細(xì)胞、Hs 888.Lu細(xì)胞、A-498細(xì)胞
SUDHL-1細(xì)胞系相關(guān)產(chǎn)品:NCI-SNU-119細(xì)胞、JIMT-1細(xì)胞、COS1細(xì)胞
CEM-T4細(xì)胞系相關(guān)產(chǎn)品:MDA-MB 468細(xì)胞、OCI-AML-2細(xì)胞、MDCK (NBL-2)細(xì)胞
NFHIOSE-80細(xì)胞系相關(guān)產(chǎn)品:Renal Proximal Tubule Epithelial Cells/TERT-immortalized 1細(xì)胞、RBL2H3細(xì)胞、COLO394細(xì)胞
PC-3細(xì)胞系相關(guān)產(chǎn)品:Lec1細(xì)胞、C57 Mouse Tumor 93細(xì)胞、H2141細(xì)胞
H295R-S1細(xì)胞系相關(guān)產(chǎn)品:AU565細(xì)胞、AN3-CA細(xì)胞、HEK-2細(xì)胞
HPAF-II/CD18細(xì)胞系相關(guān)產(chǎn)品:MSC細(xì)胞、GM-346細(xì)胞、SUP-B15細(xì)胞
HG2855細(xì)胞系相關(guān)產(chǎn)品:P116細(xì)胞、H-735細(xì)胞、MLE-12細(xì)胞
NOMO1細(xì)胞系相關(guān)產(chǎn)品:Keio University-19-19細(xì)胞、Evsa T細(xì)胞、Kit 225 K6細(xì)胞
HFLS-OA細(xì)胞系相關(guān)產(chǎn)品:HFF-1細(xì)胞、Renal Proximal Tubule Epithelial Cells/TERT-immortalized 1細(xì)胞、HGF-1細(xì)胞
CTV1細(xì)胞系相關(guān)產(chǎn)品:Intestinal Epithelioid Cell line No. 6細(xì)胞、SPC-A-1細(xì)胞、CORL23細(xì)胞
H-1341細(xì)胞系相關(guān)產(chǎn)品:A1847細(xì)胞、MDBK (NBL-1)細(xì)胞、D-341Med細(xì)胞
S.B.細(xì)胞系相關(guān)產(chǎn)品:BEL7402細(xì)胞、HEK-293-EBNA細(xì)胞、DU 145細(xì)胞
CCD 841 CoTr細(xì)胞系相關(guān)產(chǎn)品:COLO-320HSR細(xì)胞、CHP212細(xì)胞、C3H-10T1/2細(xì)胞
C42細(xì)胞系相關(guān)產(chǎn)品:LUDLU-1細(xì)胞、1E8-H細(xì)胞、PLA 802細(xì)胞
SKG3A細(xì)胞系相關(guān)產(chǎn)品:IOSE29細(xì)胞、B-3細(xì)胞、SW-48細(xì)胞
SKG3A細(xì)胞系相關(guān)產(chǎn)品:IOSE29細(xì)胞、B-3細(xì)胞、SW-48細(xì)胞
NCIH1770細(xì)胞系相關(guān)產(chǎn)品:J45.01細(xì)胞、293 F細(xì)胞、Kasumi 1細(xì)胞
H548細(xì)胞系相關(guān)產(chǎn)品:Jurkat, Clone E6-1細(xì)胞、CCD18Co細(xì)胞、TSCCa細(xì)胞
WM 2664細(xì)胞系相關(guān)產(chǎn)品:TE-9細(xì)胞、SK-MEL24細(xì)胞、MV411細(xì)胞
SW-579細(xì)胞系相關(guān)產(chǎn)品:OCI/AML3細(xì)胞、M-G63細(xì)胞、MAVER細(xì)胞
RWPE-1 Thawing人正常前列腺上皮細(xì)胞系
SK-MEL-28細(xì)胞系相關(guān)產(chǎn)品:HSF細(xì)胞、AQ-Mel細(xì)胞、4-1st細(xì)胞
Caco 2細(xì)胞系相關(guān)產(chǎn)品:HEM-L細(xì)胞、hSCC-25細(xì)胞、FRO細(xì)胞
A375S2細(xì)胞系相關(guān)產(chǎn)品:H1341細(xì)胞、PANC 1005細(xì)胞、PNT1-a細(xì)胞
HSKMC細(xì)胞系相關(guān)產(chǎn)品:C8166-CD4細(xì)胞、MD Anderson-Metastatic Breast-157細(xì)胞、SIRC細(xì)胞
38C-13細(xì)胞系相關(guān)產(chǎn)品:MAVER細(xì)胞、HT-3細(xì)胞、U343細(xì)胞
LAN-1細(xì)胞系相關(guān)產(chǎn)品:NCIH460細(xì)胞、KMM1細(xì)胞、BTI-Tn5B14細(xì)胞
P-815細(xì)胞系相關(guān)產(chǎn)品:P3-X63-Ag8細(xì)胞、Human fetal lung fibroblast 1細(xì)胞、Panc-327細(xì)胞
Porcine Kidney-13細(xì)胞系相關(guān)產(chǎn)品:H-1048細(xì)胞、P3HRI細(xì)胞、HPAF II細(xì)胞
SMMC7721細(xì)胞系相關(guān)產(chǎn)品:CTLA4Ig-24細(xì)胞、C-6細(xì)胞、GM07404細(xì)胞
NCIH647細(xì)胞系相關(guān)產(chǎn)品:Kit 225-K6細(xì)胞、Tca-83細(xì)胞、CX1細(xì)胞
H2087細(xì)胞系相關(guān)產(chǎn)品:MIN6細(xì)胞、HCC0827細(xì)胞、CCD-33Co細(xì)胞
NSH細(xì)胞系相關(guān)產(chǎn)品:THC-8307細(xì)胞、LA-N-6細(xì)胞、HEK 293 EBNA細(xì)胞
OSC-19細(xì)胞系相關(guān)產(chǎn)品:BE2_C細(xì)胞、MDA 1386細(xì)胞、CL 1-0細(xì)胞
Panc 10.05細(xì)胞系相關(guān)產(chǎn)品:U373-MG細(xì)胞、CMT-93細(xì)胞、SNUC2A細(xì)胞
SK-MEL-3細(xì)胞系相關(guān)產(chǎn)品:HBZY-1細(xì)胞、GM07404D細(xì)胞、Strain L-929細(xì)胞
H-7721細(xì)胞系相關(guān)產(chǎn)品:TSU-Pr1細(xì)胞、TGBC-11-TKB細(xì)胞、HEp-2細(xì)胞
MHH-CALL-2細(xì)胞系相關(guān)產(chǎn)品:HIEC細(xì)胞、KYSE 270細(xì)胞、OCI/AML-3細(xì)胞
Caco2BBe細(xì)胞系相關(guān)產(chǎn)品:Tn-5B1-4細(xì)胞、OVCAR4細(xì)胞、LS174T細(xì)胞
SKRC-39細(xì)胞系相關(guān)產(chǎn)品:H1155細(xì)胞、SPDC-CCL141細(xì)胞、MOLT-3細(xì)胞
SL-29細(xì)胞系相關(guān)產(chǎn)品:MB39細(xì)胞、LED-WiDr細(xì)胞、E0771細(xì)胞
Rat Skin 1細(xì)胞系相關(guān)產(chǎn)品:UCLA SO M21細(xì)胞、B16BL-6細(xì)胞、CAL39細(xì)胞
LS 1034細(xì)胞系相關(guān)產(chǎn)品:MNNG-HOS (TE 85, clone F-5)細(xì)胞、LY細(xì)胞、OVCA 433細(xì)胞
HEL 299細(xì)胞系相關(guān)產(chǎn)品:H2085細(xì)胞、MM.1S細(xì)胞、L-5178-Y細(xì)胞
OCI AML5細(xì)胞系相關(guān)產(chǎn)品:H-1693細(xì)胞、Hs-688A-T細(xì)胞、X63Ag8.653細(xì)胞
Panc_05_04細(xì)胞系相關(guān)產(chǎn)品:JIMT1細(xì)胞、H2172細(xì)胞、McARH7777細(xì)胞
HR-1細(xì)胞系相關(guān)產(chǎn)品:HEK293F細(xì)胞、Walker-256-TC細(xì)胞、SNU638細(xì)胞
Vero 76 clone E6細(xì)胞系相關(guān)產(chǎn)品:EFO 27細(xì)胞、HMCB細(xì)胞、PaTu-8988s細(xì)胞
HOP62細(xì)胞系相關(guān)產(chǎn)品:UM-RC-2細(xì)胞、SK-OV-3細(xì)胞、Ku812F細(xì)胞
Pa18C細(xì)胞系相關(guān)產(chǎn)品:HS-766T細(xì)胞、BSC40細(xì)胞、NCI-441細(xì)胞
YES 2細(xì)胞系相關(guān)產(chǎn)品:SNU520細(xì)胞、Factor Dependent Continuous-Paterson 1細(xì)胞、SUM-159細(xì)胞
SKG IIIa細(xì)胞系相關(guān)產(chǎn)品:HS578T細(xì)胞、NIH:OVCAR-3細(xì)胞、WEHI 3細(xì)胞
NCIH295R細(xì)胞系相關(guān)產(chǎn)品:TI-73細(xì)胞、NCIH1755細(xì)胞、NCI-H2591細(xì)胞
P30OHK細(xì)胞系相關(guān)產(chǎn)品:Rat-2細(xì)胞、H-4-II-E細(xì)胞、SK-MEL-5細(xì)胞
RWPE-1 Thawing人正常前列腺上皮細(xì)胞系
H2085細(xì)胞系相關(guān)產(chǎn)品:Epstein-Barr-1細(xì)胞、MC3T3, E1 subgroup-4 clone細(xì)胞、H1618細(xì)胞
3T3 L1細(xì)胞系相關(guān)產(chǎn)品:NL20SV細(xì)胞、IEC6細(xì)胞、NALM 6細(xì)胞
NW-MEL-38細(xì)胞系相關(guān)產(chǎn)品:LL/2(LLc1)細(xì)胞、D324 Med細(xì)胞、NCIH1944細(xì)胞
Tu686細(xì)胞系相關(guān)產(chǎn)品:UCLA-RO 81細(xì)胞、SUM102細(xì)胞、RPMI8402細(xì)胞
SKMEL-24細(xì)胞系相關(guān)產(chǎn)品:MDA-134細(xì)胞、AE 1201細(xì)胞、MV4II細(xì)胞
Sf21細(xì)胞系相關(guān)產(chǎn)品:U-251_MG細(xì)胞、NHRF細(xì)胞、HT-3細(xì)胞
COR-L105細(xì)胞系相關(guān)產(chǎn)品:HSC1細(xì)胞、NCIH1435細(xì)胞、Tca-8113細(xì)胞
BE(2)-C細(xì)胞系相關(guān)產(chǎn)品:NCI-H209細(xì)胞、MESSA/Dx5細(xì)胞、COLO679細(xì)胞
HuTu-80細(xì)胞系相關(guān)產(chǎn)品:Alpha Mouse Liver 12細(xì)胞、3T3-L1 ad細(xì)胞、Vero from pool #76細(xì)胞
Hs-675-T細(xì)胞系相關(guān)產(chǎn)品:HO8910細(xì)胞、C4 I細(xì)胞、RPMI-8226S細(xì)胞
H-650細(xì)胞系相關(guān)產(chǎn)品:LCLC103H細(xì)胞、ARO 81-1細(xì)胞、AtT 20細(xì)胞
"