品牌:CST，產品名稱：Beclin-1 Antibody #3738
英文名稱：Beclin-1 Antibody
Product name：Beclin-1 Antibody #3738
Source：Rabbit
Sensitivity：Endogenous
MW(kDa)：60
Reactivity：H,M,R
Applications：WB , IP ,
Purification： Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding threonine 72 of human Beclin-1. Antibodies are purified by protein A and peptide affinity chromatography.
Background： Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of proteins activated in response to nutrient deprivation and in neurodegenerative conditions (1). One of the proteins critical to this process is Beclin-1, the mammalian orthologue of the yeast autophagy protein Apg6/Vps30 (2). Beclin-1 can complement defects in yeast autophagy caused by loss of Apg6 and can also stimulate autophagy when overexpressed in mammalian cells (3). Mammalian Beclin-1 was originally isolated in a yeast two-hybrid screen for Bcl-2 interacting proteins and has been shown to interact with Bcl-2 and Bcl-xL, but not with Bax or Bak (4). While Beclin-1 is generally ubiquitously expressed, research studies have shown it is monoallelically deleted in 40-75% of sporadic human breast and ovarian cancers (5). Beclin-1 is localized within cytoplasmic structures including the mitochondria, although overexpression of Beclin-1 reveals some nuclear staining and CRM1-dependent nuclear export (6). Investigators have demonstrated that Beclin-1^-/- mice die early in embryogenesis and Beclin-1^-/+ mice have a high incidence of spontaneous tumors. Stem cells from the null mice demonstrate an altered autophagic response, although responses to apoptosis appeared normal (7). Researchers have also found that overexpression of Beclin-1 in virally infected neurons in vivo resulted in significant protection against Sindbis virus-induced disease and neuronal apoptosis (4).

Entrez-Gene Id

8678

Swiss-Prot Acc.

Q14457

Specificity / Sensitivity:

Beclin-1 Antibody detects endogenous levels of total Beclin-1 protein.

Species Reactivity:

Human, Mouse, Rat

Application	Dilutions
Western Blotting	1:1000
Immunoprecipitation	1:100

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.