Biotinylated Human TNF-alpha Protein, His,Avitag?, active trimer (MALS verified)

分子別名（Synonym）

DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Biotinylated Human TNF-alpha, His,Avitag (TNA-H82E3) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # NP_000585.2).

Predicted N-terminus: Val 77

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag?).

The protein has a calculated MW of 20.0 kDa. The protein migrates as 21 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

標(biāo)記（Labeling）

Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白標(biāo)記度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

純度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 12 months under sterile conditions after reconstitution.

背景介紹

腫瘤壞死因子α（TNFα）是一種主要由單核細(xì)胞和巨噬細(xì)胞產(chǎn)生的細(xì)胞因子，廣泛存在于組織中的滑膜細(xì)胞及巨噬細(xì)胞內(nèi)。其核心功能在于調(diào)控免疫細(xì)胞活性：可誘導(dǎo)細(xì)胞凋亡、促進(jìn)炎癥反應(yīng)、抑制腫瘤發(fā)生及病毒復(fù)制。

TNFα分泌失調(diào)與多種人類疾病相關(guān)，包括重度抑郁癥、阿爾茨海默病及惡性腫瘤。該因子的重組體（國(guó)際非專利名：tasonermin）被用作免疫刺激劑。在惡性腫瘤微環(huán)境中，TNFα可發(fā)生異位產(chǎn)生，其病理效應(yīng)與甲狀旁腺激素存在雙重相似性：既可導(dǎo)致繼發(fā)性高鈣血癥，其過度分泌亦與特定類型癌癥的發(fā)生發(fā)展密切相關(guān)。