Human CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Tag Free (MALS verified)
分子別名（Synonym）
CD3 epsilon & CD3 gamma,CD3E & CD3G
表達區(qū)間及表達系統(tǒng)（Source）
Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (CDG-H52W5) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # P07766-1 (CD3E) & P09693-1 (CD3G)).
Predicted N-terminus: Asp 23 (CD3E) & Gln 23 (CD3G)
蛋白結(jié)構(gòu)（Molecular Characterization）
Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3G, has a calculated MW of 16.9 kDa (CD3E) and 14.6 kDa (CD3G). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3G contains no tag at the C-terminus. The reducing (R) heterodimer protein migrates as 19 kDa, 21 kDa and 26-27 kDa when calibrated against Star Ribbon Pre-stained Protein Marker due to glycosylation.
內(nèi)毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
純度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景介紹
T細胞表面糖蛋白CD3δ鏈與CD3γ鏈（分別稱為CD3D與CD3G或CD3D&CD3G）屬于I型單次跨膜蛋白。CD3δ鏈與CD3ε鏈(CD3E)、CD3γ鏈、CD3ζ鏈以及T細胞受體α/β和γ/δ異源二聚體共同構(gòu)成T細胞受體-CD3復(fù)合物。該復(fù)合物在將抗原識別信號與多種細胞內(nèi)信號轉(zhuǎn)導(dǎo)通路相連接的過程中發(fā)揮關(guān)鍵作用。
關(guān)鍵字： CD3E & CD3G;CD3E蛋白;CD3G蛋白;ACRO;百普賽斯;
百普賽斯集團ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團業(yè)務(wù)遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務(wù)客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。