Human PD-1 / PDCD1 Protein, His Tag (MALS verified)

分子別名（Synonym）

PDCD1, PD1, CD279, SLEB2

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human PD-1, His Tag (PD1-H5221) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # NP_005009.2).

Predicted N-terminus: Leu 25

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 16.8 kDa. The protein migrates as 33-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 24 months in lyophilized state;

-70°C for 12 months under sterile conditions after reconstitution.

背景介紹

程序性死亡蛋白1（PD-1，亦稱(chēng)CD279或PDCD1）是一種I型跨膜蛋白，屬于T細(xì)胞調(diào)節(jié)因子CD28/CTLA-4擴(kuò)展家族成員。該蛋白表達(dá)于活化T細(xì)胞、B細(xì)胞、巨噬細(xì)胞、髓系細(xì)胞及部分胸腺細(xì)胞表面。PD-1擁有兩個(gè)B7家族配體PD-L1與PD-L2：PD-L1在幾乎所有小鼠腫瘤細(xì)胞系（包括經(jīng)IFN-γ處理的PA1骨髓瘤、P815肥大細(xì)胞瘤及B16黑色素瘤）中均有表達(dá)；PD-L2表達(dá)范圍較窄，主要存在于樹(shù)突狀細(xì)胞（DCs）及少數(shù)腫瘤細(xì)胞系。PD-1通過(guò)抑制PI3K/AKT通路活化與信號(hào)轉(zhuǎn)導(dǎo)，進(jìn)而阻遏T細(xì)胞增殖及IL-1、IL-4、IL-10和IFN-γ等細(xì)胞因子的產(chǎn)生。此外，PD-1共交聯(lián)作用可通過(guò)關(guān)鍵信號(hào)轉(zhuǎn)導(dǎo)分子去磷酸化抑制BCR介導(dǎo)的信號(hào)傳導(dǎo)。體外實(shí)驗(yàn)證實(shí)，用PD-L1-Ig融合蛋白處理抗CD3抗體刺激的T細(xì)胞可降低其增殖能力與IFN-γ分泌水平。目前基于增強(qiáng)免疫系統(tǒng)原理的PD-1靶向單克隆抗體正被開(kāi)發(fā)用于癌癥治療。