Human CD3 epsilon & CD3 delta Heterodimer Protein, His Tag&Tag Free (MALS verified)
分子別名（Synonym）
CD3E & CD3D,CD3 delta & CD3 epsilon
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）
Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (CDD-H52W1) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1 (CD3E) & P04234-1 (CD3D)).
Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)
蛋白結(jié)構(gòu)（Molecular Characterization）
Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3D, has a calculated MW of 16.6 kDa (CD3E) and 13.4 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D contains no tag. The reducing (R) heterodimer protein migrates as 25-30 kDa and 20 kDa and 22 kDa when calibrated against Star Ribbon Pre-stained Protein Marker due to glycosylation.
內(nèi)毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
純度（Purity）
>90% as determined by SDS-PAGE.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 24 months in lyophilized state;
-70°C for 12 months under sterile conditions after reconstitution.
背景介紹
T細(xì)胞表面糖蛋白CD3δ鏈與CD3ε鏈（分別簡稱CD3D與CD3E）均為I型單次跨膜蛋白。CD3D與CD3ε(CD3E)、CD3γ、CD3ζ以及T細(xì)胞受體α/β、γ/δ異源二聚體共同構(gòu)成T細(xì)胞受體-CD3復(fù)合物。該復(fù)合物在將抗原識別信號與多種胞內(nèi)信號轉(zhuǎn)導(dǎo)通路相偶聯(lián)的過程中發(fā)揮關(guān)鍵作用。
關(guān)鍵字： CD3E & CD3D;CD3E蛋白;CD3D重組蛋白;ACRO;百普賽斯;
百普賽斯集團(tuán)ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團(tuán)業(yè)務(wù)遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務(wù)客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團(tuán)旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。