Biotinylated Cynomolgus CA125 / MUC16 Protein, His,Avitag? (MALS verified)

分子別名（Synonym）

CA125,CA-125,CA125MUC-16,FLJ14303,MUC16,mucin 16,mucin-16

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Biotinylated Cynomolgus CA125 Protein, His,Avitag (CA5-C82E9) is expressed from human 293 cells (HEK293). It contains AA Pro 11544 - Pro 12188 (Accession # XP_045234963.1).

Predicted N-terminus: Pro 11544

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag?).

The protein has a calculated MW of 75.6 kDa. The protein migrates as 90-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

標(biāo)記（Labeling）

Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白標(biāo)記度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

純度（Purity）

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介紹

CA125（亦稱MUC16）是一種黏蛋白，以I型跨膜形式或分泌形式存在，被用于監(jiān)測上皮性卵巢癌的治療進(jìn)程。該分子確認(rèn)為糖蛋白結(jié)構(gòu)，以O-糖基化連接為主。其分子量和電荷均呈現(xiàn)異質(zhì)性，主要源于體液循環(huán)過程中側(cè)鏈的持續(xù)去糖基化作用。在自然狀態(tài)下，CA125以超大分子復(fù)合物形式存在（推測分子量可達(dá)400萬道爾頓）。