+ +

•  

   

     Western blot analysis of Lamin B2 in HCT116, mouse Embryo and Jurkat lysates using Lamin B2 antibody.    

  
  

•  

   

     Immunoprecipitation analysis of Lamin B2 in Hela lysates using Lamin B2 antibody.    

  
  

Lamin B2 is a key component of the nuclear lamina, a mesh-like structure underlying the inner nuclear membrane that provides mechanical support to the nucleus and regulates chromatin organization, gene expression, and cellular signaling. As a B-type lamin, Lamin B2 is distinct from A-type lamins (e.g., Lamin A/C) in its expression pattern; it is constitutively expressed in most cell types and plays critical roles during cell division, nuclear envelope reassembly, and maintaining nuclear integrity. Antibodies targeting Lamin B2 are essential tools for studying its localization, expression levels, and functional interactions in both normal and disease contexts.

These antibodies are widely used in techniques like immunofluorescence, Western blotting, and immunoprecipitation to investigate nuclear architecture defects, cell cycle dynamics, and diseases linked to lamin dysfunction. For example, altered Lamin B2 expression has been implicated in cancer progression, aging-related pathologies, and neurological disorders. Researchers also employ Lamin B2 antibodies to explore cellular senescence, as reduced Lamin B2 levels correlate with aged or stressed cells. Additionally, studies on nuclear envelope breakdown during mitosis or apoptosis often rely on these antibodies to track lamin reorganization.

Validating Lamin B2 antibody specificity is crucial, as cross-reactivity with other lamins (e.g., Lamin B1) can occur. Recent advances in super-resolution microscopy and genome editing have further expanded its applications in visualizing nuclear lamina dynamics at unprecedented resolution.