+ +

•  

   

     Western blot analysis of Phospho-DRP1 (Ser637) in Jurkat lysates using Phospho-DRP1 (Ser637) antibody.    

  
  

Phospho-DRP1 (Ser637) antibody is a specialized tool used to detect the phosphorylation status of dynamin-related protein 1 (DRP1) at serine residue 637. DRP1. a GTPase, plays a critical role in mitochondrial fission by oligomerizing around mitochondria and constricting their membranes. Its activity is tightly regulated by post-translational modifications, particularly phosphorylation. Phosphorylation at Ser637 (or Ser656 in some species, e.g., human Ser637 corresponds to mouse Ser656) inhibits DRP1’s translocation to mitochondria, thereby suppressing fission. This modification is mediated by protein kinase A (PKA) and is reversed by calcineurin-dependent dephosphorylation, linking mitochondrial dynamics to cellular energy status and calcium signaling.

Altered DRP1 phosphorylation at Ser637 is implicated in neurodegenerative diseases, cancer, and metabolic disorders, where disrupted mitochondrial dynamics contribute to pathology. Researchers use this antibody in techniques like Western blotting or immunofluorescence to assess DRP1 regulation under experimental conditions (e.g., metabolic stress, drug treatments). Specificity is crucial, as DRP1 activity is also modulated by phosphorylation at other sites (e.g., Ser616. which promotes fission). Understanding Ser637 phosphorylation provides insights into cellular homeostasis, apoptosis, and adaptive responses to stress, making this antibody valuable for studying mitochondrial biology and disease mechanisms.