+ +

•  

   

     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

•  

   

     Flow cytometric analysis of Jurkat cells using CHRM3 mouse mAb (green) and negative control (red).    

  
  

The Phospho-Rac1 (Ser71) antibody is a specialized tool for detecting Rac1. a small GTPase in the Rho family, when phosphorylated at serine 71. Rac1 plays a critical role in regulating cytoskeletal dynamics, cell migration, proliferation, and membrane trafficking. Its activity is tightly controlled by cycling between GTP-bound (active) and GDP-bound (inactive) states, as well as post-translational modifications. Phosphorylation at Ser71. mediated by kinases such as AKT or PKC, modulates Rac1 function by altering its interactions with effector proteins or regulatory guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). This modification has been linked to reduced Rac1 activity, impacting cellular processes like motility and invasiveness, particularly in cancer metastasis.

The Phospho-Rac1 (Ser71) antibody enables researchers to study this regulatory mechanism in physiological and pathological contexts. It is commonly used in techniques like Western blotting, immunofluorescence, or immunoprecipitation to assess Rac1 activation status in response to extracellular stimuli (e.g., growth factors, chemokines) or experimental manipulations. Validation typically includes testing specificity via knockout/knockdown controls or phospho-peptide competition assays. Researchers often employ this antibody to investigate signaling pathways in cancer, immune responses, or neurological disorders, where Rac1 dysregulation contributes to disease progression. Its applications extend to drug discovery, where inhibitors targeting Rac1 or its regulators are evaluated for therapeutic potential. Proper controls, such as total Rac1 detection, are essential to distinguish phosphorylation changes from expression-level variations.