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     Western blot analysis of Rb (Phospho-Thr356) in HL-60, U2OS, C6 lysates using Phospho-Rb (Thr356) antibody.    

  
  

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     Immunohistochemistry analysis of paraffin-embedded Human tonsil using Rb (Phospho-Thr356) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    

  
  

The Phospho-Rb (Thr356) antibody is a specialized tool used to detect the retinoblastoma protein (Rb) when phosphorylated at threonine 356. a post-translational modification critical for regulating cell cycle progression. Rb is a tumor suppressor protein that controls the G1-to-S phase transition by binding and inhibiting E2F transcription factors. Phosphorylation of Rb, mediated by cyclin-dependent kinases (CDKs) such as CDK4/6. disrupts this interaction, releasing E2F to activate genes required for DNA replication. Thr356 phosphorylation occurs during the mid-G1 phase and is a key marker of cell cycle commitment.

This antibody is widely used in cancer research to study Rb inactivation mechanisms, as hyperphosphorylation of Rb (due to CDK dysregulation) is a hallmark of many cancers. It helps assess cell proliferation status, drug responses (e.g., CDK4/6 inhibitors like palbociclib), and Rb signaling dynamics in diseases. Validated in techniques like Western blotting, immunofluorescence, and immunohistochemistry, the antibody’s specificity for the phosphorylated Thr356 epitope ensures accurate detection of active Rb inactivation. Its applications extend to understanding developmental biology, apoptosis, and therapeutic resistance, making it essential for studies linking cell cycle control to oncogenesis or targeted therapies.