+ +

•  

   

     Western blot analysis of Cdk2 (Phospho-Thr14) in Hela, A549, HL-60, U2OS lysates using Phospho-CDK2 (Thr14) antibody.    

  
  

•  

   

     Immunohistochemistry analysis of paraffin-embedded Human tonsil using Cdk2 (Phospho-Thr14) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    

  
  

The Phospho-CDK2 (Thr14) antibody is a specialized tool used to detect the phosphorylation of cyclin-dependent kinase 2 (CDK2) at threonine residue 14. a post-translational modification critical for regulating CDK2 activity. CDK2 plays a central role in cell cycle progression, particularly during the G1-to-S phase transition, by forming complexes with cyclins E and A. Phosphorylation at Thr14. along with Tyr15. inhibits CDK2 kinase activity, serving as a regulatory mechanism to prevent premature entry into DNA replication. This inhibitory modification is reversed by phosphatases like CDC25 during cell cycle checkpoints, ensuring precise timing of phase transitions.

Researchers use the Phospho-CDK2 (Thr14) antibody primarily in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to study cell cycle dynamics, checkpoint control, and DNA damage responses. Its specificity for the phosphorylated Thr14 epitope allows differentiation between active and inactive CDK2 states. This antibody is particularly valuable in cancer research, as dysregulated CDK2 activity is linked to uncontrolled proliferation. Studies also explore its role in neurodegenerative diseases and developmental disorders. Proper experimental controls, including non-phosphorylated samples or Thr14-to-alanine mutants, are essential to validate signal specificity. Handling requires preservation of phosphorylation states via phosphatase inhibitors during sample preparation.