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     Western blot analysis of Phospho-AKT1 (Thr450) in U2OS, C6 lysates using Phospho-AKT1 (Thr450) antibody.    

  
  

**Background of Phospho-AKT1 (Thr450) Antibody**

The Phospho-AKT1 (Threonine 450) antibody is a specialized tool used to detect AKT1 protein phosphorylated at the Thr450 residue, a post-translational modification critical for regulating AKT1 activity. AKT1 (Protein Kinase B) is a serine/threonine kinase central to the PI3K/AKT/mTOR signaling pathway, which governs cellular processes such as survival, proliferation, metabolism, and apoptosis. Phosphorylation at Thr450 occurs in the catalytic domain of AKT1 and is associated with its maturation and stabilization. Unlike the canonical activation loop phosphorylation (Thr308/Ser473), Thr450 phosphorylation is not directly linked to immediate kinase activation but is thought to play a role in proper protein folding during AKT1 synthesis, ensuring its functional integrity.

This antibody is widely utilized in research to investigate AKT1 regulation, particularly in contexts of cancer, diabetes, and neurodegenerative diseases, where aberrant AKT signaling is often implicated. It enables the detection of phosphorylated AKT1 in various experimental setups, including Western blotting, immunofluorescence, and immunohistochemistry. Validation typically involves testing in cell lines with induced phosphorylation (e.g., growth factor stimulation) or genetic/pharmacologic inhibition. Researchers rely on this antibody to dissect AKT1 dynamics, assess pathway activation status, and evaluate therapeutic responses in preclinical models. Specificity for the Thr450 site ensures minimal cross-reactivity with other AKT isoforms (AKT2/AKT3) or unrelated phosphoproteins, making it a critical reagent for precision studies in signal transduction.