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     Western blot analysis of PREB expression in Raji cell lysate.    

  
  

PREB (Prolactin Regulatory Element-Binding protein) is a transcription factor initially identified for its role in regulating prolactin gene expression by binding to a specific element in the prolactin promoter. It belongs to the WD40 repeat protein family, characterized by conserved β-propeller structures that mediate protein-protein interactions. PREB functions as a regulatory component in secretory pathways, influencing processes like vesicle trafficking and hormone secretion, particularly in pituitary cells. Beyond prolactin regulation, it has been implicated in broader cellular functions, including endoplasmic reticulum stress response, lipid metabolism, and adipocyte differentiation.

PREB antibodies are essential tools for studying its expression, localization, and molecular interactions. These antibodies are typically developed in hosts like rabbits or mice, targeting specific epitopes within PREB's N-terminal or C-terminal regions. They are widely used in techniques such as Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate PREB's role in transcriptional regulation and cellular homeostasis. Dysregulation of PREB has been linked to metabolic disorders, obesity, and certain cancers, making its antibodies valuable for both basic research and clinical investigations. Validation of PREB antibodies includes testing for specificity using knockout cell lines or siRNA-mediated knockdown to ensure accurate detection in diverse biological samples.