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     Western blot analysis of RASSF2 expression in Jurkat cell lysate.    

  
  

The RASSF2 antibody is a crucial tool for studying the Ras association domain family member 2 (RASSF2), a protein encoded by the tumor suppressor gene *RASSF2*. Belonging to the RASSF family, RASSF2 contains a Ras association (RA) domain and a SARAH domain, enabling interactions with Ras-related GTPases and other signaling molecules. It plays a role in regulating apoptosis, cell cycle arrest, and Hippo signaling pathways. Dysregulation of RASSF2 has been linked to tumorigenesis, as its promoter hypermethylation and subsequent silencing are frequently observed in cancers like colorectal, lung, and prostate cancers, suggesting its role as a tumor suppressor.

RASSF2 antibodies are designed to detect endogenous RASSF2 protein levels in various experimental setups, including Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies aid in elucidating RASSF2's expression patterns, post-translational modifications, and interactions with signaling partners. Polyclonal or monoclonal RASSF2 antibodies are validated for specificity using knockout cell lines or siRNA-mediated knockdown controls. Their applications extend to both basic research—exploring RASSF2's mechanistic roles in cancer progression—and translational studies, such as evaluating its potential as a diagnostic or prognostic biomarker. Proper validation ensures reliability in distinguishing RASSF2 isoforms and avoiding cross-reactivity with other RASSF family members.