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     Western blot analysis of ORP1 expression in 293 cell lysate.    

  
  

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

ORP1 (Oxysterol-binding protein-related protein 1), also known as OSBPL1. is a member of the oxysterol-binding protein (OSBP) family, which plays critical roles in lipid metabolism, intracellular lipid transport, and cellular signaling. ORP1 is characterized by its ability to bind oxysterols, cholesterol, and phospholipids, facilitating their distribution between organelles, particularly between the endoplasmic reticulum (ER) and late endosomes/lysosomes. It contains a pleckstrin homology (PH) domain and a FFAT motif, enabling interactions with membrane surfaces and vesicle-associated membrane proteins. ORP1 is implicated in regulating cholesterol homeostasis, endosomal trafficking, and autophagy, with dysregulation linked to diseases such as cancer, neurodegenerative disorders, and metabolic syndromes.

ORP1 antibodies are essential tools for studying the protein’s expression, localization, and function. These antibodies are commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect ORP1 in cellular or tissue samples. Researchers utilize them to explore ORP1’s role in lipid-mediated signaling pathways, its interaction with molecular partners (e.g., VAP proteins and RAB7A), and its involvement in disease mechanisms. Validated ORP1 antibodies help clarify its dual role in promoting cholesterol export from endosomes and modulating mTORC1 activity, linking lipid metabolism to cellular growth and stress responses. Their specificity and sensitivity are critical for distinguishing ORP1 isoforms and understanding its tissue-specific functions.