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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of BANF1 expression in HeLa cell lysate.    

  
  

The BANF1 antibody is a crucial tool in studying the Barrier-to-Autointegration Factor 1 (BANF1), a highly conserved protein involved in nuclear membrane assembly, chromatin organization, and DNA repair. BANF1 binds to DNA and lamin-associated proteins, playing a key role in maintaining nuclear architecture during cell division and post-mitotic nuclear reformation. Dysregulation of BANF1 is linked to genetic disorders like Nestor-Guillermo progeria syndrome and autoimmune diseases, as well as viral infection mechanisms (e.g., HIV).

The antibody is widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to detect BANF1 expression, localization, and interactions. It helps researchers investigate its role in cell cycle regulation, nuclear envelope dynamics, and DNA damage response. Commercial BANF1 antibodies are typically raised in rabbits or mice, validated for specificity using knockout controls or siRNA knockdown. Cross-reactivity varies, with many antibodies recognizing human, mouse, and rat isoforms.

Recent studies also utilize BANF1 antibodies to explore its involvement in cancer progression, aging-related pathologies, and as a potential biomarker. Proper validation remains critical due to BANF1's low molecular weight (~10 kDa) and potential cross-reactivity with unrelated proteins.