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     Western blot analysis of TIP49A expression in K562 cell lysate.    

  
  

TIP49A antibody targets the TIP49A protein, also known as RUVBL1 (RuvB-like AAA ATPase 1), a conserved member of the AAA+ (ATPases Associated with Diverse Cellular Activities) superfamily. TIP49A plays critical roles in chromatin remodeling, transcriptional regulation, and DNA repair by facilitating ATP-dependent processes. It interacts with various multiprotein complexes, including the INO80 and SWR1 chromatin remodelers, and the TIP60 histone acetyltransferase complex, influencing nucleosome positioning, histone variant exchange, and gene expression. TIP49A also participates in cell cycle regulation, telomere maintenance, and oncogenic signaling pathways, with dysregulation linked to cancers, such as colorectal and breast malignancies.

The TIP49A antibody is a vital tool for studying these processes. It is commonly used in Western blotting, immunoprecipitation, and immunofluorescence to detect protein expression, localization, and interactions in cellular or tissue samples. Researchers employ it to investigate TIP49A’s functional partnerships (e.g., with TIP49B/RUVBL2) and its role in diseases. Validated antibodies ensure specificity for TIP49A, distinguishing it from related AAA+ proteins. Its applications extend to cancer research, where TIP49A’s overexpression or mutation is explored as a potential therapeutic target. Overall, this antibody supports mechanistic insights into chromatin dynamics, genome stability, and disease pathogenesis.