+ +

•  

   

     Western blot analysis of Pumilio 2 expression in 293T cell lysate.    

  
  

Pumilio2 (PUM2) is an RNA-binding protein belonging to the conserved PUF (Pumilio/FBF) family, which plays critical roles in post-transcriptional regulation of gene expression by binding to specific sequences in the 3' untranslated regions (3'UTRs) of target mRNAs. Initially identified in *Drosophila* for its role in embryonic development and germline stem cell maintenance, mammalian PUM2 is implicated in diverse processes, including neurodevelopment, synaptic plasticity, and cellular stress responses. It regulates mRNA stability, translation, and localization, often collaborating with other RNA-binding proteins (RBPs) like FMRP or Nanos.

PUM2 antibodies are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques such as Western blotting, immunofluorescence, and RNA immunoprecipitation (RIP) to investigate PUM2's role in neuronal function, stem cell dynamics, and diseases like cancer or neurodegeneration. Commercial PUM2 antibodies are typically raised against conserved regions (e.g., the N-terminal or RNA-binding PUF domain) and validated for specificity using knockout controls. Studies have linked PUM2 dysregulation to glioblastoma progression, Alzheimer’s disease, and spermatogenesis defects, highlighting its therapeutic relevance. However, cross-reactivity with PUM1. a closely related paralog, remains a technical consideration. Recent advances in CLIP-seq and CRISPR-based approaches have expanded PUM2 research, revealing its context-dependent regulatory networks in health and disease.