The U2AF65 antibody targets the U2AF65 protein (U2 snRNP Auxiliary Factor 65 kDa), a critical component of the pre-mRNA splicing machinery. U2AF65. encoded by the *U2AF2* gene, is a conserved splicing factor that recognizes the polypyrimidine tract near the 3' splice site of introns, facilitating the assembly of the spliceosome by recruiting U2 snRNP to pre-mRNA. It forms a heterodimer with U2AF35 (U2AF1) to stabilize interactions with splice sites, ensuring accurate splicing. Dysregulation of U2AF65 is linked to splicing errors implicated in cancers, neurodegenerative diseases, and genetic disorders. The U2AF65 antibody is widely used in molecular biology research to study splicing mechanisms, RNA-protein interactions, and cellular localization via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Its specificity makes it a valuable tool for investigating splicing anomalies in disease models and elucidating post-transcriptional gene regulation. Commercial U2AF65 antibodies are typically validated in multiple applications to ensure reliability in detecting endogenous protein levels across various experimental systems.