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     Western blot analysis of NCF1 expression in Raji cell lysate.    

  
  

The NCF1 (Neutrophil Cytosolic Factor 1) antibody targets the NCF1 protein, a critical subunit of the NADPH oxidase complex essential for reactive oxygen species (ROS) production in phagocytes. NCF1. also known as p47phox, plays a pivotal role in host defense by enabling immune cells like neutrophils and macrophages to generate antimicrobial ROS during the "respiratory burst." Mutations in the NCF1 gene are linked to chronic granulomatous disease (CGD), a rare immunodeficiency disorder characterized by recurrent bacterial and fungal infections due to impaired ROS-dependent pathogen clearance.

NCF1 antibodies are widely used in research to study the expression, localization, and functional regulation of the NADPH oxidase complex. They aid in diagnosing CGD by detecting protein deficiencies via Western blotting, flow cytometry, or immunofluorescence. Additionally, these antibodies contribute to investigations into oxidative stress-related pathologies, including inflammatory diseases, autoimmune disorders, and cancer, where dysregulated ROS signaling is implicated.

Commercially available NCF1 antibodies are typically raised in rabbits or mice, targeting specific epitopes of the human or murine protein. Their applications span basic research, clinical diagnostics, and therapeutic development, helping elucidate molecular mechanisms of immune dysfunction and potential targets for modulating oxidative stress pathways.