The NPRC (Natrium Potassium Chloride Cotransporter) antibody is a crucial tool in studying ion transport mechanisms, particularly targeting the NKCC1 and NKCC2 proteins encoded by the SLC12A family. These transporters regulate cellular electrolyte balance by facilitating Na?-K?-2Cl? symport across membranes. NKCC1 is widely expressed in secretory epithelia, neurons, and smooth muscle, playing roles in fluid secretion, cell volume regulation, and neuroexcitability. NKCC2. found predominantly in the kidney’s thick ascending limb, is essential for renal salt reabsorption and blood pressure regulation. NPRC antibodies enable detection, localization, and functional analysis of these transporters via techniques like Western blot, immunohistochemistry, and immunofluorescence. Research applications include investigating pathologies such as hypertension, epilepsy, and cystic fibrosis, where dysregulated ion transport is implicated. Additionally, these antibodies aid in exploring the pharmacodynamics of loop diuretics (e.g., furosemide), which target NKCC2. Developed in hosts like rabbits or mice, NPRC antibodies are validated for specificity through knockdown/knockout controls. Their utility spans physiology, pharmacology, and clinical research, offering insights into electrolyte homeostasis and therapeutic targeting.