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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     Immunofluorescent analysis using the Antibody at 1:50 dilution.    

  
  

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     Immunofluorescent analysis using the Antibody at 1:100 dilution.    

  
  

Fructose-6-phosphate kinase (F6P kinase) antibodies are immunological tools designed to detect and study fructose-6-phosphate kinases, a group of enzymes critical to carbohydrate metabolism. These enzymes primarily catalyze the phosphorylation of fructose-6-phosphate (F6P) to fructose-1.6-bisphosphate (F1.6BP), a key regulatory step in glycolysis. Two major isoforms, phosphofructokinase-1 (PFK-1) and phosphofructokinase-2 (PFK-2), are often targeted. PFK-1 drives glycolysis, while PFK-2 regulates glycolytic flux via synthesis of fructose-2.6-bisphosphate, an allosteric activator of PFK-1. Dysregulation of these enzymes is linked to metabolic disorders, cancer, and diabetes.

F6P kinase antibodies are generated using immunogenic peptides or recombinant proteins from conserved regions of the enzymes, ensuring specificity for Western blotting, immunohistochemistry, or ELISA. They aid in quantifying protein expression, assessing post-translational modifications, or localizing enzymes in tissues. Monoclonal antibodies offer high reproducibility, whereas polyclonal versions may detect multiple isoforms across species. Researchers employ these antibodies to investigate metabolic adaptations in diseases, such as tumorigenesis (via the Warburg effect) or insulin resistance. Validation includes knockout controls or enzymatic activity assays to confirm functional correlations. Commercial antibodies often target human, mouse, or rat isoforms, with applications spanning basic research and drug development targeting metabolic pathways.