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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of xCT expression in (1) HepG2 cell lysate; (2) Mouse brain lysate.    

  
  

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     Immunofluorescent analysis using the Antibody at 1:50 dilution.    

  
  

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     Immunofluorescent analysis using the Antibody at 1:50 dilution.    

  
  

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     Immunofluorescent analysis using the Antibody at 1:150 dilution.    

  
  

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     Immunofluorescent analysis using the Antibody at 1:150 dilution.    

  
  

The xCT antibody targets the xCT protein, a key subunit of the system xc? cystine/glutamate antiporter encoded by the *SLC7A11* gene. This transporter facilitates the exchange of intracellular glutamate with extracellular cystine, a critical step in maintaining cellular redox balance. Cystine is reduced to cysteine, a precursor for glutathione synthesis, a major antioxidant that protects cells from oxidative stress. xCT’s role in regulating redox homeostasis links it to ferroptosis, a form of iron-dependent cell death triggered by lipid peroxidation. Dysregulation of xCT has been implicated in cancer, neurodegenerative disorders, and inflammatory diseases.

In cancer, xCT is often overexpressed, enabling tumor cells to resist oxidative stress and chemotherapy. It also modulates the tumor microenvironment by exporting glutamate, which may promote tumor progression via paracrine signaling. In neurodegenerative conditions like Alzheimer’s disease, disrupted system xc? activity may exacerbate excitotoxicity due to excessive glutamate release. xCT antibodies are widely used in research to detect protein expression levels via techniques like Western blotting, immunohistochemistry, or flow cytometry, aiding studies on disease mechanisms or therapeutic targeting. Recent interest in xCT inhibitors as ferroptosis inducers highlights its potential as a biomarker or therapeutic target in precision medicine. However, antibody specificity and validation remain critical to avoid off-target artifacts in experimental models.